Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5891 |
| PDBID: | 8CW9 |
| Chains: | CD_A |
| Organism: | human metapneumovirus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.46 |
| Reference: | 10.1016/j.immuni.2022.07.003 |
| Antibody | |
| Antibody: | ADI-6102 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Prefusion-stabilized human metapneumovirus (hMPV) fusion protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >8CW9_C|Chain C, H, I|ADI-61026 heavy|Homo sapiens (9606) QVQLVQSGPALVKSTQTLTLTCTFSGFALTTSGMCVSWVRQPPGKALEWLARIDWEDNTYYSTSLKTRVTISKDPSKNQVVLTMTNMDPVDTATYYCARSYITDWKKDWFFDLWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: D
Mutation: NULL
| >8CW9_D|Chain D, K, L|ADI-61026 light|Homo sapiens (9606) SYELIQLPSVSVSPGQTARIPCSGDGLPKKYAHWYQQKAGQAPILLMYKDSERPSGIPERFSAFSSGTTVTMTISGVQEEDEADYYCQSGDSTDTSVIFGGGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: Q100R/S101R/L110C/T127C/A140C/A147C/N153C/A185P/L219K/V231I/N322C/T365C/E453Q/V463C
| >8CW9_A|Chain A, E, F|Fusion glycoprotein F0|Human metapneumovirus (162145) MSWKVVIIFSLLITPQHGLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCADGPSLIKTELDLTKSALRELRTVSADQLAREEQIENPRRRRFVLGAIACGVATAAAVTAGVAIAKCIRLESEVTAIKNCLKKTNECVSTLGCGVRVLATAVRELKDFVSKNLTRAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISKDLMTDAELARAISNMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSEKKGNYACLLREDQGWYCQNAGSTVYYPCEKDCETRGDHVFCDTAAGINVAEQSKECNINISTTNYPCKVSCGRHPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPVKFPQDQFNVALDQCFESIENSQALVDQSNRILSSAEKGNTGGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER32 TRP53 GLU54 THR98 ASP99 D: LEU28 PRO29 LYS30 LYS31 TYR32 LYS50 ASP51 SER66 THR94 A: ASP54 ASN57 LEU58 THR59 CYS60 ILE67 LEU71 LYS166 VAL169 SER170 LEU173 THR174 ARG175 ILE177 ASN178 LYS179 ASN180 LYS181 CYS182 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
