Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5890 |
| PDBID: | 8CT6 |
| Chains: | HL_A |
| Organism: | Influenza A virus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.10 |
| Reference: | 10.1038/s42003-023-04793-3 |
| Antibody | |
| Antibody: | 1F8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus Computationally optimized broadly reactive antigen (COBRA) H1N1 P1 hemagglutinin (HA) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8CT6_H|Chain E[auth H], G[auth J]|1F8 heavy chain|Mus musculus (10090) QVQLQQSGAELMKPGASVKISCKATGYTFSSYWIEWVKQRPGHGLEWIGEILPGSGRTNYDERFKGKATFTADTSSNTAYMQLSSLTSEDSAVYYCARPRIYGMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIEPRGLVPR |
Light Chain: L
Mutation: NULL
| >8CT6_L|Chain D[auth K], F[auth L]|1F8 light chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCKASQSVDFDGDTYMSWYQQKPGQPPKLLIYAASKLESGIPARFSGSGSGTDFTLNIHPVEEEDAATYFCQQSNEDPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >8CT6_A|Chain A, B, C|COBRA P1 HA|Influenza A virus (11320) DTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCKLKGIAPLQLGKCNIAGWLLGNPECESLLSARSWSYIVETPNSENGTCYPGDFIDYEELREQLSSVSSFERFEIFPKESSWPNHNTTKGVTAACSHAGKSSFYRNLLWLTKKGGSYPKLSKSYVNNKGKEVLVLWGVHHPSTSTDQQSLYQNENAYVSVVSSNYNRRFTPEIAERPKVRGQAGRMNYYWTLLEPGDTIIFEATGNLIAPWYAFALSRGSGSGIITSNASMHECNTKCQTPQGAINSSLPFQNIHPVTIGECPKYVRSTKLRMVTGLRNIPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNSVIEKMNTQFTAVGKEFNNLEKRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLRNNAKEIGNGCFEFYHKCDNECMESVKNGTYDYPKYSEESKLNREKIDGVKLESMGVYGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGLNDIFEAQKIEWHEGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASP30 TYR32 ASN92 TRP96 H: SER31 TYR32 TRP33 GLU35 GLU50 SER54 PRO95 ARG96 ILE97 TYR98 A: GLU68 TYR91 LYS130 VAL132 THR133 ALA134 SER137 HIS138 ALA139 GLY140 LYS141 SER142 TRP150 THR152 HIS180 PRO182 SER183 ASP187 SER190 LEU191 GLY222 GLN223 GLY225 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
