Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5888 |
| PDBID: | 8CML |
| Chains: | C_B |
| Organism: | Lama glama, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.60 |
| Reference: | 10.1016/j.jbc.2023.104956 |
| Antibody | |
| Antibody: | UNbC5-1 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human complement C5 alpha chain |
| Antigen mutation: | No |
| Durg Target: | P01031; P01031; |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >8CML_C|Chain D[auth C]|Nanobody UNbC5-1|Lama glama (9844) MEVQLVESGGGLVQAGGSLRLSCAASGFTFDDYAIGWFRQAPGKEREGVSCISTSDGSTYYADSVKGRFTISSDNAKNTVYLQMNSLKPEDTAVYYCAADPYLPIRGRGIESTDFGSWGQGTQVTVSSAAASGSLEQKLISEEDLNGAAHHHHHHGAA |
Antigen
Chain: B
Mutation: NULL
| >8CML_B|Chain C[auth B]|Complement C5 alpha chain|Homo sapiens (9606) TLQKKIEEIAAKYKHSVVKKCCYDGACVNNDETCEQRAARISLGPRCIKAFTECCVVASQLRANISHKDMQLGRLHMKTLLPVSKPEIRSYFPESWLWEVHLVPRRKQLQFALPDSLTTWEIQGVGISNTGICVADTVKAKVFKDVFLEMNIPYSVVRGEQIQLKGTVYNYRTSGMQFCVKMSAVEGICTSESPVIDHQGTKSSKCVRQKVEGSSSHLVTFTVLPLEIGLHNINFSLETWFGKEILVKTLRVVPEGVKRESYSGVTLDPRGIYGTISRRKEFPYRIPLDLVPKTEIKRILSVKGLLVGEILSAVLSQEGINILTHLPKGSAEAELMSVVPVFYVFHYLETGNHWNIFHSDPLIEKQKLKKKLKEGMLSIMSYRNADYSYSVWKGGSASTWLTAFALRVLGQVNKYVEQNQNSICNSLLWLVENYQLDNGSFKENSQYQPIKLQGTLPVEARENSLYLTAFTVIGIRKAFDICPLVKIDTALIKADNFLLENTLPAQSTFTLAISAYALSLGDKTHPQFRSIVSALKREALVKGNPPIYRFWKDNLQHKDSSVPNTGTARMVETTAYALLTSLNLKDINYVNPVIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLRLSMDIDVSYKHKGALHNYKMTDKNFLGRPVEVLLNDDLIVSTGFGSGLATVHVTTVVHKTSTSEEVCSFYLKIDTQDIEASHYRGYGNSDYKRIVACASYKPSREESSSGSSHAVMDISLPTGISANEEDLKALVEGVDQLFTDYQIKDGHVILQLNSIPSSDFLCVRFRIFELFEVGFLSPATFTVYEYHRPDKQCTMFYSTSNIKIQKVCEGAACKCVEADCGQMQEELDLTISAETRKQTACKPEIAYAYKVSITSITVENVFVKYKATLLDIYKTGEAVAEKDSEITFIKKVTCTNAELVKGRQYLIMGKEALQIKYNFSFRYIYPLDSLTWIEYWPRDTTCSSCQAFLANLDEFAEDIFLNGC |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP31 CYS50 SER52 SER54 ASP55 SER57 THR58 ASP99 PRO100 TYR101 LEU102 PRO103 ILE104 ARG105 GLY106 ARG107 SER111 ASP113 B: ARG849 THR850 SER851 GLY852 MET853 GLN854 PHE855 CYS856 LYS858 SER880 CYS883 ARG885 LYS887 VAL888 GLU889 GLU915 THR916 TRP917 PHE918 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
