Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 588 |
| PDBID: | 3MXW |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.83 |
| Reference: | 10.1074/jbc.M110.112284 |
| Antibody | |
| Antibody: | 5E1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Sonic hedgehog protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3MXW_H|Chain C[auth H]|5E1 heavy chain|Homo sapiens (9606) QVQLQQSGPELVRPGVSVKISCKGSGYTFIDEALHWVKQSHAESLEWIGVIRPYSGETNYNQKFKDKATMTVDISSSTAYLELARLTSEDSAIYYCARDWERGDFFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: L
Mutation: NULL
| >3MXW_L|Chain B[auth L]|5E1 light chain|Homo sapiens (9606) DIVMTQTPKFLLVSAGDKVTITCKASQSVSNDLTWYQQKPGQSPKLLIYYASNRYTGVPDRFTGSGYGTDFTFTISTVQAEDLAVYFCQQDYGSPPTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >3MXW_A|Chain A|Sonic hedgehog protein|Homo sapiens (9606) GFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition L: SER30 ASN31 ASP32 TYR49 TYR50 ASN53 ARG54 TYR55 THR56 TYR67 ASP91 TYR92 GLY93 SER94 H: ASP31 GLU32 ARG52 TYR54 ASP95 TRP96 GLU97 ARG98 GLY99 A: TYR44 LYS45 LYS87 GLU89 ARG123 THR125 GLU126 ASP131 GLY132 HIS133 HIS134 SER135 GLU136 ASP147 THR149 ASP152 ARG153 ARG155 TYR158 TYR174 GLU176 SER177 LYS178 ALA179 HIS180 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
