Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5873 |
| PDBID: | 8CE4 |
| Chains: | H_C |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | EM |
| Resolution (Å): | 2.70 |
| Reference: | 10.1038/s41467-023-41734-4 |
| Antibody | |
| Antibody: | E3 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human neuronal acetylcholine receptor subunit alpha-7 |
| Antigen mutation: | No |
| Durg Target: | P36544; Q494W8; |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >8CE4_H|Chain F, G, H, I, J|Nanobody E3|Vicugna pacos (30538) AVQLQASGGGLVQAGDSLRLSCAASGGTFSHYAVGWFRQAPGKEREFVAAISWSGRSTSFANSVKGRFTISRDSAKNTAYLQMNNLKPEDTAVYCCAPARFGTGSAARDEYDDCGQGTQVTVSSAAAEQKLISEEDLNGAAHHHHHHGS |
Antigen
Chain: C
Mutation: NULL
| >8CE4_C|Chain A, B, C, D, E|Neuronal acetylcholine receptor subunit alpha-7|Homo sapiens (9606) EFQRKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRDGVAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFASAGLTETSQVAPA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: VAL2 GLY26 GLY27 THR28 HIS31 TYR32 TRP53 SER54 ARG56 ARG100 PHE101 GLY102 ASP109 GLU110 ASP112 C: GLU9 LEU10 LYS12 ASN13 ALA22 ASN23 SER25 HIS62 TYR63 GLN65 TRP66 ASN67 GLU70 TYR71 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
