Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 587 |
| PDBID: | 3MJ9 |
| Chains: | HL_A |
| Organism: | Mus musculus, Nothocricetulus migratorius |
| Method: | XRD |
| Resolution (Å): | 2.95 |
| Reference: | 10.1016/j.str.2010.10.007 |
| Antibody | |
| Antibody: | HL4E10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Junctional adhesion molecule-like |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3MJ9_H|Chain C[auth H]|STIMULATORY HAMSTER ANTIBODY HL4E10 FAB HEAVY CHAIN|CRICETULUS MIGRATORIUS (3122392) QVQLKESGPGLLQPSQTLSLTCTVSGISLSDYGVHWVRQAPGKGLEWMGIIGHAGGTDYNSNLKSRVSISRDTSKSQVFLKLNSLQQEDTAMYFCARHFYTYFDVWGQGIQVTVSSATTTAPSVYPLAPACDSTTSTTNTVTLGCLVKGYFPEPVTVSWNSGALTSGVHTFPSVLHSGLYSLSSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPGDGSGC |
Light Chain: L
Mutation: NULL
| >3MJ9_L|Chain B[auth L]|STIMULATORY HAMSTER ANTIBODY HL4E10 FAB LIGHT CHAIN|CRICETULUS MIGRATORIUS (3122392) SYTLTQPPLVSVALGQKATITCSGDKLSDVYVHWYQQKAGQAPVLVIYEDNRRPSGIPDHFSGSNSGNMATLTISKAQAGDEADYYCQSWDGTNSAWVFGSGTKVTVLGQPNAAPSVTLFPPSSEELKTNQATLVCMINGFYPADVAVTWEADGTPITQGVKTTQPSKSDSKYMATSYLTMTADAWKSRNTFICKVTHGGNTVEKSLSPSACS |
Antigen
Chain: A
Mutation: NULL
| >3MJ9_A|Chain A|Junctional adhesion molecule-like|Mus musculus (10090) RSQGLPGLTVSSPQLRVHVGESVLMGCVVQRTEEKHVDRVDWLFSKDKDDASEYVLFYYSNLSVPTGRFQNRSHLVGDTFHNDGSLLLQDVQKADEGIYTCEIRLKNESMVMKKPVELWVLPEEPRDLRVRVGDTTQMRCSIQSTEEKRVTKVNWMFSSGSHTEEETVLSYDSNMRSGKFQSLGRFRNRVDLTGDISRNDGSIKLQTVKESDQGIYTCSIYVGKLESRKTIVLHVVQDEFQRTISPTPPTDKGQQGILNGNQHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: SER29 ASP30 VAL31 TYR32 TYR49 GLU50 ASP51 ARG53 ARG54 SER56 TRP91 TRP96 H: GLN1 VAL2 THR23 SER25 GLY26 ILE27 SER28 ASP31 TYR32 GLY33 ILE50 GLY52 HIS53 GLY56 THR57 ASP58 LYS75 SER76 ARG94 HIS95 PHE96 TYR97 THR98 TYR99 ASP101 VAL102 A: GLN28 ARG29 THR30 GLU31 ASP76 HIS79 GLU145 LYS146 VAL148 THR149 LYS150 TYR169 ASP170 SER171 ASN172 MET173 ARG174 SER175 GLY176 LYS177 PHE178 LEU190 THR191 GLY192 ASP193 ILE194 SER195 ARG196 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
