Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5865 |
| PDBID: | 8CAU |
| Chains: | G_B |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | EM |
| Resolution (Å): | 3.40 |
| Reference: | 10.1038/s41467-023-41734-4 |
| Antibody | |
| Antibody: | C4 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human neuronal acetylcholine receptor subunit alpha-7 |
| Antigen mutation: | No |
| Durg Target: | P36544; Q494W8; |
Sequence information
Antibody
Chain: G
Mutation: NULL
| >8CAU_G|Chain F, G, H, I, J|Nanobody C4|Vicugna pacos (30538) AQVQLVESGGGLVQAGGSLKLSCAASGFTFAHYAMVWFRQAPGKEREFVAGISWSGASTYYASSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYVAAARFGVGVDDDYSYWGQGTQVTVSSAAEQKLISEEDLNGAAHHHHHHGS |
Antigen
Chain: B
Mutation: NULL
| >8CAU_B|Chain A, B, C, D, E|Neuronal acetylcholine receptor subunit alpha-7|Homo sapiens (9606) EFQRKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFASAGLTETSQVAPA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: GLY27 PHE28 THR29 HIS32 TYR33 TRP54 ARG101 PHE102 ASP108 ASP109 TYR110 SER111 TYR112 B: GLU9 LEU10 LYS12 ASN13 ALA22 HIS62 TYR63 GLN65 ASN67 GLU70 TYR71 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
