Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5851 |
| PDBID: | 8BLQ |
| Chains: | EC_D |
| Organism: | Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.97 |
| Reference: | 10.1038/s41598-023-35678-4 |
| Antibody | |
| Antibody: | RefAb Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human interleukin-13 |
| Antigen mutation: | No |
| Durg Target: | P35225; P35225; |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >8BLQ_E|Chain E|RefAb, heavy chain|Homo sapiens (9606) VQLVQSGAEVKKPGASVKVSCKASGYTFTNYGLSWVRQAPGQGLEWMGWISANNGDTNYGQEFQGRVTMTTDTSTSTAYMELRSLRSDDTAVYYCARDSSSSWARWFFDLWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: C
Mutation: NULL
| >8BLQ_C|Chain C|RefAb, light chain|Homo sapiens (9606) YVLTQPPSVSVAPGKTARITCGGNIIGSKLVHWYQQKPGQAPVLVIYDDGDRPSGIPERFSGSNSGNTATLTISRVEAGDEADYYCQVWDTGSDPVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTEC |
Antigen
Chain: D
Mutation: NULL
| >8BLQ_D|Chain D|Interleukin-13, human|Homo sapiens (9606) PPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVSGCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFREGQFN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ILE26 GLY28 SER29 LYS30 LEU31 HIS33 ASP49 ASP50 GLY51 ASP52 ASN65 SER66 GLY67 ASN68 THR69 TRP90 THR92 E: TRP104 ALA105 ARG106 TRP107 PHE108 D: SER25 ARG29 ILE32 GLU33 VAL36 THR39 GLN40 ASN41 GLN42 LYS115 LEU118 LEU119 LYS122 LYS123 PHE125 ARG126 GLU127 GLY128 PHE130 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
