Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5847 |
| PDBID: | 8BK2 |
| Chains: | HL_A |
| Organism: | Neisseria meningitidis serogroup B, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.41 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | 1B1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Neisseria meningitidis serogroup B meningococcal factor H binding protein variant 2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8BK2_H|Chain D, F, H|Fab Heavy chain|Homo sapiens (9606) QPAMAEVQLVESGGNLVQPGGSLRLSCAASGFTFSSYSMNWVRQAPGKGLEWVSYISSSSSTIYYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARVAAGLKDWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDK |
Light Chain: L
Mutation: NULL
| >8BK2_L|Chain E, G, I[auth L]|Fab light Chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSISSSYLAWYQQKPGQAPRLLIFGASSRATGIPDRFSGSGSGTGFTLTISRLEPEDFAVYYCQHYDSSLPMTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >8BK2_A|Chain A, B, C|Factor H-binding protein|Neisseria meningitidis serogroup B (491) GPDSDRLQQRRVAADIGAGLADALTAPLDHKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGEFQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELAAAELKADEKSHAVILGDTRYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIGIAGKQLE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 SER33 TYR34 SER35 TYR52 SER54 SER55 SER56 SER58 ARG100 VAL101 ALA102 ALA103 LYS106 L: SER32 TYR33 PHE50 GLY51 SER53 SER54 ARG55 THR57 GLY65 SER66 TYR92 SER94 SER95 A: PHE145 ASN146 ASP150 LYS152 ASP174 ALA176 ALA177 LYS178 GLN179 HIS181 GLU195 ALA197 ALA198 ALA199 GLU200 LEU201 LYS202 ASP214 ARG216 SER219 GLU221 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
