Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5836 |
| PDBID: | 8ATH |
| Chains: | EF_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.37 |
| Reference: | 10.1080/19420862.2023.2175311 |
| Antibody | |
| Antibody: | B Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human lysosome-associated membrane glycoprotein 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >8ATH_E|Chain C[auth E], E[auth H]|Fab B Heavy Chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGYIFTNYNIHWVKKSPGQGLEWIGAIYPGNGDAPYSQKFQGKATLTADTSTSTTYMELSSLRSEDTAVYYCVRANWDVAFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTASHHHHHH |
Light Chain: F
Mutation: NULL
| >8ATH_F|Chain D[auth F], F[auth L]|Fab B Light Chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCKASQDIDRYMAWYQDKPGKAPRLLIHDTSTLQSGVPSRFSGSGSGRDYTLTISNLEPEDFATYYCLQYDNLWTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >8ATH_B|Chain A, B|Lysosome-associated membrane glycoprotein 1|Homo sapiens (9606) GSHMAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: GLY26 ILE28 THR30 ASN31 TYR32 ASN33 TYR52 ASN55 ASP57 PRO59 ASN100 TRP101 ASP102 PHE105 F: ASP30 ARG31 TYR32 ASP50 ASP92 LEU94 B: ASN35 ASN37 CYS41 CYS80 GLY81 THR105 ARG106 ASN107 ALA108 THR109 ILE149 ASP150 LYS151 TYR178 LEU179 SER180 ASN181 ARG186 GLY187 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
