Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5835 |
| PDBID: | 8AS3 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Phage display vector pTDisp |
| Method: | XRD |
| Resolution (Å): | 3.50 |
| Reference: | 10.1016/j.molcel.2023.05.002 |
| Antibody | |
| Antibody: | 30 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human arrestin 2 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >8AS3_H|Chain C[auth H]|Fab30 heavy chain|Homo sapiens (9606) VQLVESGGGLVQPGGSLRLSCAASGFNVYSSSIHWVRQAPGKGLEWVASISSYYGYTYYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSRQFWYSGLDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHHHHHHHH |
Light Chain: L
Mutation: NULL
| >8AS3_L|Chain D[auth L]|Fab30 light chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYKYVPVTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECEISEV |
Antigen
Chain: A
Mutation: C150L/C242V/C251V/C269S
| >8AS3_A|Chain A|Beta-arrestin-1|Homo sapiens (9606) MGDKGTRVFKKASPNGKLTVYLGKRDFVDHIDLVDPVDGVVLVDPEYLKERRVYVTLTCAFRYGREDLDVLGLTFRKDLFVANVQSFPPAPEDKKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGKACGVDYEVKAFLAENLEEKIHKRNSVRLVIRKVQYAPERPGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQYADIVLFNTAQYKVPVAMEEADDTVAPSSTFSKVYTLTPFLANNREKRGLALDGKLKHEDTNLASSTLLREGANREILGIIVSYKVKVKLVVSRGGLLGDLASSDVAVELPFTLMHPKPKEE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN31 TYR33 SER34 SER56 TYR57 TYR58 GLY59 TYR60 ARG103 PHE105 TRP106 L: SER32 TYR50 ARG67 GLY69 A: ARG7 ARG169 TYR173 TYR209 HIS210 GLY211 GLU212 PRO213 THR275 PRO276 PHE277 LEU278 ALA279 ARG282 ASP290 ASP297 THR298 ASN299 LEU300 HIS353 PRO354 PRO356 LYS357 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
