Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5813 |
| PDBID: | 8A1E |
| Chains: | BC_A |
| Organism: | Rabies virus strain Pasteur vaccin, Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.83 |
| Reference: | 10.1016/j.chom.2022.07.014 |
| Antibody | |
| Antibody: | 1112-1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Rabies virus glycoprotein |
| Antigen mutation: | No |
| Durg Target: | P08667; P08667; |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >8A1E_B|Chain E[auth B]|Fab 1112-1 heavy chain variable domain|Mus musculus (10090) QVQLQQPGAELLKPGTSMKLSCKASGYTFSNYWMHWVKLRPGQGFEWIGEINPFNGGTNFNEKFKSKATLTVDRSSSTAYMQLSSVTSEDSAVYYCTIPLSDYGDWFFAVWGAGTTVTVSS |
Light Chain: C
Mutation: NULL
| >8A1E_C|Chain D[auth C]|Fab 1112-1 light chain variable domain|Mus musculus (10090) DIVMTQSHKFMSTSIGDRVSITCKASQDVSTAVAWYRQKPGQSPKLLIYSASYRSTGVPDRFTGSGSGTDFTFTISSVQAEDLAVFYCQQHYSAPLTFGAGTKLELK |
Antigen
Chain: A
Mutation: NULL
| >8A1E_A|Chain A|Glycoprotein|Rabies virus strain Pasteur vaccin (103929) KFPIYTIPDKLGPWSPIDIHHLSCPNNLVVEDEGCTNLSGFSYMELKVGYISAIKMNGFTCTGVVTEAETYTNFVGYVTTTFKRKHFRPTPDACRAAYNWKMAGDPRYEESLHNPYPDYHWLRTVKTTKESLVIISPSVADLDPYDRSLHSPVFPGGNCSGVAVSSTYCSTNHDYTIWMPENPRLGMSCDIFTNSRGKRASKGSETCGFVDERGLYKSLKGACKLKLCGVLGLRLMDGTWVAMQTSNETKWCPPGQLVNLHDFRSDEIEPLVVEELVKKREECLDALESIMTTKSVSFRRLSHLRKLVPGFGKAYTIFNKTLMEADAHYKSVRTWNEIIPSKGCLRVGGRCHPHVNGVFFNGIILGPDGNVLIPEMQSSLLQQHMELLVSSVIPLMHPLADPSTVFKNGDEAEDFVEVHLPDVHERISGVDLGLPNWGKYVLLSAGALTALMLIIFLMTCWRRVNRSEPTQHNLRGTGREVSVTPQSGKIISSWESHKSGGETRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER30 THR31 ALA32 TYR49 SER50 TYR53 HIS91 TYR92 SER93 ALA94 B: SER30 ASN31 TYR32 TRP33 GLU50 ASN52 PHE54 ASN55 GLY57 ASN59 ARG74 LEU100 SER101 ASP102 TYR103 GLY104 ASP105 TRP106 PHE107 A: THR36 ASN37 LEU38 SER39 GLY40 PHE41 SER42 TYR43 MET44 CYS189 ASP190 PHE192 ASN194 SER195 ARG196 GLY197 LYS198 ARG199 SER201 LYS202 GLY203 SER204 CYS228 GLY229 VAL230 LEU231 ALA242 MET243 GLN244 THR245 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
