Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5812 |
| PDBID: | 8A1E |
| Chains: | DE_A |
| Organism: | Rabies virus strain Pasteur vaccin, Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.83 |
| Reference: | 10.1016/j.chom.2022.07.014 |
| Antibody | |
| Antibody: | 17C7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Rabies virus glycoprotein |
| Antigen mutation: | No |
| Durg Target: | P08667; P08667; |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >8A1E_D|Chain B[auth D]|Fab 17C7 heavy chain variable domain|Homo sapiens (9606) EVQLVESGGGVVQPGRSLRLSCAASGFTFSTYAMHWVRQAPGKGLEWVAVVSYDGRTKDYADSVKGRFTISRDNSKNTLYLQMNSLRTEDTAVYFCARERFSGAYFDYWGQGTLVTVSS |
Light Chain: E
Mutation: NULL
| >8A1E_E|Chain C[auth E]|Fab 17C7 light chain variable domain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPARFSGSGSGTDFTLTISSLEPEDFAVYSCQQRNNWPPTFGGGTKVEIKRA |
Antigen
Chain: A
Mutation: NULL
| >8A1E_A|Chain A|Glycoprotein|Rabies virus strain Pasteur vaccin (103929) KFPIYTIPDKLGPWSPIDIHHLSCPNNLVVEDEGCTNLSGFSYMELKVGYISAIKMNGFTCTGVVTEAETYTNFVGYVTTTFKRKHFRPTPDACRAAYNWKMAGDPRYEESLHNPYPDYHWLRTVKTTKESLVIISPSVADLDPYDRSLHSPVFPGGNCSGVAVSSTYCSTNHDYTIWMPENPRLGMSCDIFTNSRGKRASKGSETCGFVDERGLYKSLKGACKLKLCGVLGLRLMDGTWVAMQTSNETKWCPPGQLVNLHDFRSDEIEPLVVEELVKKREECLDALESIMTTKSVSFRRLSHLRKLVPGFGKAYTIFNKTLMEADAHYKSVRTWNEIIPSKGCLRVGGRCHPHVNGVFFNGIILGPDGNVLIPEMQSSLLQQHMELLVSSVIPLMHPLADPSTVFKNGDEAEDFVEVHLPDVHERISGVDLGLPNWGKYVLLSAGALTALMLIIFLMTCWRRVNRSEPTQHNLRGTGREVSVTPQSGKIISSWESHKSGGETRL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: THR31 TYR32 ALA33 VAL50 SER52 TYR53 ASP54 ARG56 THR57 ASP59 ASP62 GLU99 ARG100 PHE101 SER102 GLY103 ALA104 TYR105 E: GLU1 GLN27 SER28 TYR32 ASP50 ARG91 ASN92 ASN93 TRP94 PRO95 A: ASP9 GLU212 ARG213 GLY214 LYS330 ARG333 THR334 TRP335 ASN336 GLU337 ILE339 PRO340 SER341 LYS342 CYS344 ARG346 GLY348 GLY349 ARG350 CYS351 ASN370 VAL371 MET376 LEU380 GLN382 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
