Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5808 |
| PDBID: | 7ZWM |
| Chains: | IJ_F |
| Organism: | Plasmodium falciparum, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.69 |
| Reference: | 10.1038/s41467-022-33379-6 |
| Antibody | |
| Antibody: | 32F3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum gametocyte surface protein P45/48 C-terminal domain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >7ZWM_I|Chain D, I|10D8 heavy chain|Mus musculus (10090) MNFGLSLIFLVLVLKGVQCEVMLVESGGDLVKPGGSLKVSCAASGFTFSNYAMSWVRQTPEKRLEWVATISSGASYTHYPDSVKGRFTISRDNAKNTLYLQMSSLRSEDTAMYYCGRQVNRHDRALDAMDYWGQGTSVTVSPAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK |
Light Chain: J
Mutation: NULL
| >7ZWM_J|Chain E, J|10D8 light chain|Mus musculus (10090) MDSQAQVLMLLLLWVSGTCGDIVMSQSPSSLAVSVGEKVTMSCKSSQSLFYSSNQKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVKAEDLAVYYCQQYYSYPPTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: F
Mutation: NULL
| >7ZWM_F|Chain A, F|Gametocyte surface protein P45/48|Plasmodium falciparum (5833) MMLYISAKKAQVAFILYIVLVLRIISGNNDFCKPSSLNSEISGFIGYKCNFSNEGVHNLKPDMRERRSIFCTIHSYFIYDKIRLIIPKKSSSPEFKILPEKCFQKVYTDYENRVETDISELGLIEYEIEENDTNPNYNERTITISPFSPKDIEFFCFCDNTEKVISSIEGRSAMVHVRVLKYPHNILFTNLTNDLFTYLPKTYNESNFVSNVLEVELNDGELFVLACELINKKCFQEGKEKALYKSNKIIYHKNLTIFKAPFYVTSKDVNTECTCKFKNNNYKIVLKPKYEKKVIHGCNFSSNVSSKHTFTDSLDISLVDDSAHISCNVHLSEPKYNHLVGLNCPGDIIPDCFFQVYQPESEELEPSNIVYLDSQINIGDIEYYEDAEGDDKIKLFGIVGSIPKTTSFTCICKKDKKSAYMTVTIDSA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: SER30 SER52 SER53 GLY54 ALA55 SER56 TYR57 THR58 HIS59 TYR60 THR69 ILE70 ASP104 ARG105 ALA106 LEU107 J: TYR31 ASN34 TYR38 TYR98 SER99 TYR100 F: TYR203 ASN204 GLU205 SER206 ASN207 PHE208 VAL209 SER210 GLU214 VAL215 GLU216 LYS267 VAL285 LYS287 PRO288 LYS289 TYR290 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
