Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5805 |
| PDBID: | 7ZWM |
| Chains: | BC_A |
| Organism: | Plasmodium falciparum, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.69 |
| Reference: | 10.1038/s41467-022-33379-6 |
| Antibody | |
| Antibody: | 10D8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum gametocyte surface protein P45/48 C-terminal domain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7ZWM_B|Chain B, G|32F3 heavy chain|Mus musculus (10090) DVKLVESGGGLVKLGGSLKLSCAASGFTFSSYYMSWVRQTPEKRLELVAAINNNGGSTYYPDTVKGRFTISRDNAKNTLNLQMNSLKSEDTALYYCTRQHYGNLYFFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLESDLYTLSSSVTVPSSPWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMNTNGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK |
Light Chain: C
Mutation: NULL
| >7ZWM_C|Chain C, H|32F3 light chain|Mus musculus (10090) QIVLSQSPAILSASPGEKVTMTCRASSSVTYIHWYQQKPGSSPKPWIQATSSLASGVPARFSGSGSGTSYSLSISRVEAEDAATYYCQQWSSNPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >7ZWM_A|Chain A, F|Gametocyte surface protein P45/48|Plasmodium falciparum (5833) MMLYISAKKAQVAFILYIVLVLRIISGNNDFCKPSSLNSEISGFIGYKCNFSNEGVHNLKPDMRERRSIFCTIHSYFIYDKIRLIIPKKSSSPEFKILPEKCFQKVYTDYENRVETDISELGLIEYEIEENDTNPNYNERTITISPFSPKDIEFFCFCDNTEKVISSIEGRSAMVHVRVLKYPHNILFTNLTNDLFTYLPKTYNESNFVSNVLEVELNDGELFVLACELINKKCFQEGKEKALYKSNKIIYHKNLTIFKAPFYVTSKDVNTECTCKFKNNNYKIVLKPKYEKKVIHGCNFSSNVSSKHTFTDSLDISLVDDSAHISCNVHLSEPKYNHLVGLNCPGDIIPDCFFQVYQPESEELEPSNIVYLDSQINIGDIEYYEDAEGDDKIKLFGIVGSIPKTTSFTCICKKDKKSAYMTVTIDSA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: VAL2 PHE27 TYR32 TYR33 ALA50 ILE51 ASN52 ASN53 SER57 THR58 TYR59 ARG98 HIS100 TYR101 GLY102 ASN103 LEU104 TYR105 ASP108 TYR109 C: THR30 TYR31 GLN48 ALA49 SER52 LEU53 ALA54 SER55 TRP90 SER91 SER92 ASN93 A: SER322 ALA323 HIS324 ASP347 ILE349 TYR357 GLN358 GLU360 SER361 GLU362 GLU363 LEU364 GLU365 PRO366 SER367 ASN368 ILE369 THR409 ILE411 LYS413 ASP415 LYS416 SER418 TYR420 THR422 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
