Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5802 |
| PDBID: | 7ZWF |
| Chains: | B_A |
| Organism: | Plasmodium falciparum, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.13 |
| Reference: | 10.1038/s41467-022-33379-6 |
| Antibody | |
| Antibody: | 32F3 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum gametocyte surface protein P45/48 full length |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7ZWF_B|Chain B|32F3scFv|Mus musculus (10090) MGILPSPGMPALLSLVSLLSVLLMGCVAETGDVKLVESGGGLVKLGGSLKLSCAASGFTFSSYYMSWVRQTPEKRLELVAAINNNGGSTYYPDTVKGRFTISRDNAKNTLNLQMNSLKSEDTALYYCTRQHYGNLYFFDYWGQGTTLTVSGGSSRSSSSGGGGSGGGGQIVLSQSPAILSASPGEKVTMTCRASSSVTYIHWYQQKPGSSPKPWIQATSSLASGVPARFSGSGSGTSYSLSISRVEAEDAATYYCQQWSSNPLTFGAGTKLELKGTKHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7ZWF_A|Chain A|Gametocyte surface protein P45/48|Plasmodium falciparum (5833) MMLYISAKKAQVAFILYIVLVLRIISGNNDFCKPSSLNSEISGFIGYKCNFSNEGVHNLKPDMRERRSIFCTIHSYFIYDKIRLIIPKKSSSPEFKILPEKCFQKVYTDYENRVETDISELGLIEYEIEENDTNPNYNERTITISPFSPKDIEFFCFCDNTEKVISSIEGRSAMVHVRVLKYPHNILFTNLTNDLFTYLPKTYNESNFVSNVLEVELNDGELFVLACELINKKCFQEGKEKALYKSNKIIYHKNLTIFKAPFYVTSKDVNTECTCKFKNNNYKIVLKPKYEKKVIHGCNFSSNVSSKHTFTDSLDISLVDDSAHISCNVHLSEPKYNHLVGLNCPGDIIPDCFFQVYQPESEELEPSNIVYLDSQINIGDIEYYEDAEGDDKIKLFGIVGSIPKTTSFTCICKKDKKSAYMTVTIDSA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: ASP32 VAL33 TYR63 TYR64 ALA81 ILE82 ASN83 ASN84 ASN85 SER88 THR89 TYR90 ARG129 HIS131 TYR132 GLY133 ASN134 LEU135 TYR136 ASP139 TYR140 THR198 TYR199 GLN216 ALA217 SER220 LEU221 ALA222 SER223 TRP258 SER259 SER260 ASN261 A: ASP321 SER322 ALA323 HIS324 ASP347 ILE349 TYR357 GLN358 GLU360 SER361 GLU362 GLU363 LEU364 GLU365 PRO366 SER367 ASN368 ILE369 THR409 ILE411 LYS413 ASP415 LYS416 SER418 TYR420 THR422 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
