Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5798 |
| PDBID: | 7ZRA |
| Chains: | F_C |
| Organism: | Escherichia coli, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1016/j.str.2022.09.004 |
| Antibody | |
| Antibody: | SOS1 (14497) Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Escherichia coli lexA repressor |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >7ZRA_F|Chain C[auth E], D[auth F], G, H|Nanobody NbSOS1 (Nb14497)|Lama glama (9844) QVQLVESGGGSVQAGGSLRLSCAASGSIFSINAMGWYRQAPGKQRELVAAITRRGSTNYADFVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCKARIEPDSSWGTEYEYWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: C
Mutation: NULL
| >7ZRA_C|Chain A, B, E[auth C], F[auth D]|LexA repressor|Escherichia coli (562) MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: GLN1 VAL2 SER27 PRO101 SER103 SER104 TYR109 GLU110 TYR111 C: ASN163 LEU181 ARG182 GLN183 GLN184 SER185 PHE186 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
