Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5788 |
| PDBID: | 7ZMK |
| Chains: | VW_X |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | AS0326 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human microfibril-associated glycoprotein 4 (MFAP4) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: V
Mutation: NULL
| >7ZMK_V|Chain B, E, G, J, M, P, S, V|heavy chain of antibody AS0326|Mus musculus (10090) QMQLVQSGPEVKKPGTSVKVSCKASGFTFTSYWMHWVRQARGQRLEWIGVIHPNSGNTKYNEKFRSRVTMTTDTSTSTAYMELRSLRSDDTAVYYCAREMWNYGNSWYFDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: W
Mutation: NULL
| >7ZMK_W|Chain C, F, H, K, N, Q, T, W|Light chain of AS0326|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCRASKSISKYLAWYQQKPGKAPELLIYSGSTLQSGIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQHNEYPFTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: X
Mutation: NULL
| >7ZMK_X|Chain A, D, I, L, O, R, U, X|Microfibril-associated glycoprotein 4|Homo sapiens (9606) MKALLALPLLLLLSTPPCAPQVSGIRGDALERFCLQQPLDCDDIYAQGYQSDGVYLIYPSGPSVPVPVFCDMTTEGGKWTVFQKRFNGSVSFFRGWNDYKLGFGRADGEYWLGLQNMHLLTLKQKYELRVDLEDFENNTAYAKYADFSISPNAVSAEEDGYTLFVAGFEDGGAGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFWFRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| V: SER31 TRP33 VAL50 HIS52 ASN54 SER55 ASN57 LYS59 GLU99 TRP101 ASN102 TYR103 GLY104 ASN105 SER106 TRP107 W: SER30 LYS31 TYR32 ASN92 TYR94 X: TYR24 ALA25 GLN26 GLY27 TYR28 GLN29 SER30 MET51 THR52 GLU54 GLY55 LYS57 GLN103 GLU106 TYR120 LYS122 GLU148 ARG232 ARG233 ALA234 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
