Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 575 |
| PDBID: | 3LEV |
| Chains: | HL_A |
| Organism: | Thermus aquaticus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1073/pnas.1004728107 |
| Antibody | |
| Antibody: | 2F5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | RNA polymerase sigma factor |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3LEV_H|Chain C[auth H]|2F5 ANTIBODY HEAVY CHAIN|Homo sapiens (9606) RITLKESGPPLVKPTQTLTLTCSFSGFSLSDFGVGVGWIRQPPGKALEWLAIIYSDDDKRYSPSLNTRLTITKDTSKNQVVLVMTRVSPVDTATYFCAHRRGPTTLFGVPIARGPVNAMDVWGQGITVTISSTSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >3LEV_L|Chain B[auth L]|2F5 ANTIBODY LIGHT CHAIN|Homo sapiens (9606) ALQLTQSPSSLSASVGDRITITCRASQGVTSALAWYRQKPGSPPQLLIYDASSLESGVPSRFSGSGSGTEFTLTISTLRPEDFATYYCQQLHFYPHTFGGGTRVDVRRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >3LEV_A|Chain A|RNA polymerase sigma factor|Thermus aquaticus (271) SDPVRQYLHEIGEVLELDKWAELGAAAKVEEGMEAIKKLSEATGLDQELIREVVRAKILGTAAIQKIPGLKEKPDPKTVEEVDGKLKSLPKELKRYLHIAREGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKFEYKRGFAFSTYATWWIRQAINRAIADQAR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ALA1 LEU2 GLN27 LEU91 HIS92 PHE93 TYR94 PRO95 HIS96 H: PHE32 GLY33 TYR52 ASP54 ASP56 ARG58 TYR59 PRO61 ARG95 PRO98 A: GLU105 LEU107 GLU108 LEU109 ASP110 LYS111 TRP112 ALA113 GLU114 LEU115 GLY116 ASN206 TYR244 LYS245 ALA249 SER251 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
