Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5703 |
| PDBID: | 7YZI |
| Chains: | E_A |
| Organism: | Mycobacterium tuberculosis '98-R604 INH-RIF-EM, Vicugna pacos |
| Method: | EM |
| Resolution (Å): | 3.83 |
| Reference: | 10.7554/eLife.77032 |
| Antibody | |
| Antibody: | NB4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mycobacterium tuberculosis adenylyl cyclase Rv1625c / Cya |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: E
Mutation: NULL
| >7YZI_E|Chain C, D, E|Nanobody Nb4|Vicugna pacos (30538) MAQWQLVESGGGLVQAGGSLRLSCTASGIILSINSMGWYRQTAGNEREWVAFSTAGGSTTYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNTPAGRVGGTWGQGTPVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7YZI_A|Chain A, B|Adenylate cyclase|Mycobacterium tuberculosis '98-R604 INH-RIF-EM' (555461) MGSAWSHPQFEKGSGLEVLFQGPSGHMAARKCGAPPIAADGSTRRPDCVTAVRTQARAPTQHYAESVARRQRVLTITAWLAVVVTGSFALMQLATGAGGWYIALINVFTAVTFAIVPLLHRFGGLVAPLTFIGTAYVAIFAIGWDVGTDAGAQFFFLVAAALVVLLVGIEHTALAVGLAAVAAGLVIALEFLVPPDTGLQPPWAMSVSFVLTTVSACGVAVATVWFALRDTARAEAVMEAEHDRSEALLANMLPASIAERLKEPERNIIADKYDEASVLFADIVGFTERASSTAPADLVRFLDRLYSAFDELVDQHGLEKIKVSGDSYMVVSGVPRPRPDHTQALADFALDMTNVAAQLKDPRGNPVPLRVGLATGPVVAGVVGSRRFFYDVWGDAVNVASRMESTDSVGQIQVPDEVYERLKDDFVLRERGHINVKGKGVMRTWYLIGRKVAADPGEVRGAEPRTAGVAAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition E: SER38 TYR42 GLU49 ARG50 TRP52 PHE55 THR57 THR63 PRO103 ALA104 ARG106 VAL107 GLY108 TRP111 A: THR122 ASP123 PRO168 PRO169 ASP170 THR171 GLY172 LEU173 GLN174 PRO175 PRO176 TRP177 MET179 SER180 PHE183 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
