Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5701 |
| PDBID: | 7YZI |
| Chains: | D_A |
| Organism: | Mycobacterium tuberculosis '98-R604 INH-RIF-EM, Vicugna pacos |
| Method: | EM |
| Resolution (Å): | 3.83 |
| Reference: | 10.7554/eLife.77032 |
| Antibody | |
| Antibody: | NB4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mycobacterium tuberculosis adenylyl cyclase Rv1625c / Cya |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7YZI_D|Chain C, D, E|Nanobody Nb4|Vicugna pacos (30538) MAQWQLVESGGGLVQAGGSLRLSCTASGIILSINSMGWYRQTAGNEREWVAFSTAGGSTTYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNTPAGRVGGTWGQGTPVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7YZI_A|Chain A, B|Adenylate cyclase|Mycobacterium tuberculosis '98-R604 INH-RIF-EM' (555461) MGSAWSHPQFEKGSGLEVLFQGPSGHMAARKCGAPPIAADGSTRRPDCVTAVRTQARAPTQHYAESVARRQRVLTITAWLAVVVTGSFALMQLATGAGGWYIALINVFTAVTFAIVPLLHRFGGLVAPLTFIGTAYVAIFAIGWDVGTDAGAQFFFLVAAALVVLLVGIEHTALAVGLAAVAAGLVIALEFLVPPDTGLQPPWAMSVSFVLTTVSACGVAVATVWFALRDTARAEAVMEAEHDRSEALLANMLPASIAERLKEPERNIIADKYDEASVLFADIVGFTERASSTAPADLVRFLDRLYSAFDELVDQHGLEKIKVSGDSYMVVSGVPRPRPDHTQALADFALDMTNVAAQLKDPRGNPVPLRVGLATGPVVAGVVGSRRFFYDVWGDAVNVASRMESTDSVGQIQVPDEVYERLKDDFVLRERGHINVKGKGVMRTWYLIGRKVAADPGEVRGAEPRTAGVAAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ILE33 SER35 ILE36 ASN37 SER38 TYR42 TRP52 PHE55 THR57 ALA58 GLY59 SER61 THR63 ASN78 PRO103 ALA104 ARG106 VAL107 GLY108 A: ALA282 GLU285 LEU286 ASP288 GLN289 HIS290 GLY291 ARG312 ALA318 ASP321 ASP325 ASN328 VAL329 GLN332 LEU333 ARG424 LYS425 VAL426 ALA428 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
