Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5700 |
| PDBID: | 7YZ9 |
| Chains: | B_A |
| Organism: | Mycobacterium tuberculosis H37Rv, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 1.97 |
| Reference: | 10.7554/eLife.77032 |
| Antibody | |
| Antibody: | NB4 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mycobacterium tuberculosis adenylyl cyclase (AC) Rv1625c/Cya |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7YZ9_B|Chain B|nanobody NB4|Vicugna pacos (30538) MAQWQLVESGGGLVQAGGSLRLSCTASGIILSINSMGWYRQTAGNEREWVAFSTAGGSTTYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNTPAGRVGGTWGQGTPVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7YZ9_A|Chain A|Adenylate cyclase|Mycobacterium tuberculosis H37Rv (83332) MGHHHHHHHHHHSSGLEVLFQGPSGHMRDTARAEAVMEAEHDRSEALLANMLPASIAERLKEPERNIIADKYDEASVLFADIVGFTERASSTAPADLVRFLDRLYSAFDELVDQHGLEKIKVSGDSYMVVSGVPRPRPDHTQALADFALDMTNVAAQLKDPRGNPVPLRVGLATGPVVAGVVGSRRFFYDVWGDAVNVASRMESTDSVGQIQVPDEVYERLKDDFVLRERGHINVKGKGVMRTWYLIGRKVAAD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ILE33 SER35 ILE36 ASN37 SER38 TYR42 TRP52 PHE55 THR57 ALA58 GLY59 SER61 THR63 PRO103 ALA104 ARG106 VAL107 GLY108 A: ARG82 ALA86 GLU89 LEU90 ASP92 GLN93 HIS94 GLY95 ARG116 ASP118 ASP125 ASP129 ASN132 VAL133 GLN136 LEU137 ARG228 LYS229 VAL230 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
