Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 568 |
| PDBID: | 3KS0 |
| Chains: | KJ_A |
| Organism: | Mus musculus, Saccharomyces cerevisiae |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.jmb.2010.05.035 |
| Antibody | |
| Antibody: | B2B4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | mitochondrial cytochrome b2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: K
Mutation: NULL
| >3KS0_K|Chain B[auth H], E[auth K]|Fragment Antigen Binding B2B4|Mus musculus (10090) EVQLQESGPSLVKPSQTLSLTCSVTGDSITSGYWNWIRKFPGNKLEYMGYISYGGSTYYNPSLESRISITRDTSKNQYYLQLNSVTTEDTATYFCARLFGSYYFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCI |
Light Chain: J
Mutation: NULL
| >3KS0_J|Chain A[auth J], D[auth L]|heme domain of flavocytochrome b2|Mus musculus (10090) QAVVTQESALTTSPGETVTLTCRSSTGAVTTSNYANWVQEKPDHLFTGLIGGTNKRAPGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWDSNHLVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLSPADC |
Antigen
Chain: A
Mutation: NULL
| >3KS0_A|Chain C[auth A], F[auth B]|Cytochrome b2, mitochondrial|Saccharomyces cerevisiae (4932) MNKQKISPAEVAKHNKPDDCWVVINGYVYDLTRFLPNHPGGQDVIKFNAGKDVTAIFEPLHAPNVIDKYIAPEKKLGPLQGSMPPELVCPPYAPG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: THR31 SER32 TYR34 LYS55 TRP93 ASN96 K: SER31 TYR33 TYR50 TYR53 SER56 THR57 TYR58 LEU98 PHE99 GLY100 SER101 TYR102 A: ILE29 ASN30 ALA60 PHE62 GLU63 PRO64 LEU65 HIS66 ALA67 PRO68 ASN69 VAL70 ASP72 LYS73 TYR74 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
