Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 566 |
| PDBID: | 3KR3 |
| Chains: | HL_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1158/1535-7163.MCT-09-1134 |
| Antibody | |
| Antibody: | DX-2647 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Insulin-like growth factor II |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3KR3_H|Chain B[auth H]|antibody-Fab (heavy chain)|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSNYIMWWVRQAPGKGLEWVSVISSSGGMTRYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCARDNGDYVGEKGFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCAAAHHHHHHGAAEQKLISEEDLNGAA |
Light Chain: L
Mutation: NULL
| >3KR3_L|Chain C[auth L]|antibody-Fab (light chain)|Homo sapiens (9606) QDIQMTQSPSSLSASVGDRVTITCRASQSISNYLNWYQQKPGKAPKLLIYTASTLQSGVPSRFSGSASGTDFTLTINSLQPEDFATYSCQQSYNSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTQEDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >3KR3_D|Chain A[auth D]|Insulin-like growth factor II|Homo sapiens (9606) AYRPSETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPAKSE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 ASN31 TYR32 ILE33 VAL50 ILE51 SER52 SER53 SER54 GLY56 MET57 THR58 ARG59 GLY101 ASP102 TYR103 VAL104 GLU106 L: SER31 TYR33 SER92 TYR93 ASN94 SER95 TRP97 D: SER5 THR7 CYS9 GLY10 GLY11 GLU12 LEU13 VAL14 ASP15 LEU17 GLN18 PHE26 PRO31 ARG34 VAL35 ARG37 SER39 ARG40 GLY41 VAL43 GLU44 GLU45 CYS47 PHE48 ARG49 TYR59 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
