Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5623 |
| PDBID: | 7XRZ |
| Chains: | BA_Y |
| Organism: | Mus musculus, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1107/S205979832300311X |
| Antibody | |
| Antibody: | SRP2070 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Escherichia coli soluble cytochrome b562: BRIL |
| Antigen mutation: | Yes |
| Durg Target: | P0ABE7 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7XRZ_B|Chain B, E[auth H]|IGG HEAVY CHAIN|Mus musculus (10090) QIQLQQSGPELVKPGASVKISCKASGYTFTDFYINWMKQRPGQGLEWIGWIFPGSGNTHYNEKFKGKATLIVDTSSSTAFMQLNSLTSEDSAVYFCTRPVSYYYDFDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTSGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGP |
Light Chain: A
Mutation: NULL
| >7XRZ_A|Chain A, D[auth L]|IGG LIGHT CHAIN|Mus musculus (10090) DIVLTQSPATLSVTPGDRVSLSCRASQSVSNYLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVETEDFGMYFCQQSNSWPLTFGAGTKLELRRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: Y
Mutation: M7W/H102I/R106L
| >7XRZ_Y|Chain C[auth X], F[auth Y]|Soluble cytochrome b562|Escherichia coli (562) GDLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TYR32 TYR50 SER91 ASN92 SER93 TRP94 LEU96 B: THR28 THR30 ASP31 PHE32 TYR33 ASN35 TRP50 PHE52 GLY54 SER55 ASN57 THR58 HIS59 PRO99 VAL100 SER101 TYR102 TYR103 TYR104 Y: GLU18 HIS63 ILE67 GLY70 GLN71 ASP73 ASP74 LYS77 LEU78 GLU81 LYS83 LYS85 GLU86 GLN88 ALA89 ALA90 GLU92 GLN93 LYS95 THR96 THR97 ARG98 ASN99 ALA100 TYR101 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
