Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 561 |
| PDBID: | 3K81 |
| Chains: | A_D |
| Organism: | Trypanosoma brucei, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1016/j.jsb.2010.10.007 |
| Antibody | |
| Antibody: | 3K81 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MP18 RNA editing complex protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >3K81_A|Chain C[auth A], D[auth B]|Single strand antibody VHH domain|Lama glama (9844) EVQPQESGGGLAQAGGSLRLSCVVSGITFASEAWGWYRRAPGKQRELIAAINNEGRTNYVDSVKGRFTVSRDNAKNVMYLQMNSLKPEDTAVYYCNANLQTGTLSGARLYWGQGTQVTVSSHHHHHH |
Antigen
Chain: D
Mutation: NULL
| >3K81_D|Chain A[auth C], B[auth D]|MP18 RNA editing complex protein|Trypanosoma brucei (5691) MLALTSRRLLLQQTFMRCCKSVNSVTLVGVVHDIQSGFVYEDAVTQFTLTTTSIDTTHPTQEVVVEKDHHTIRCFGELFSAEVKQKVKEGNVVCVNGRLRLSPQLEPSCNKHFYFPYIQVQPPHGQVAVIHGDRRTVPAAVNPAVEDIKSEKEGSGGDQSGVPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ALA33 LEU47 ALA50 ILE51 ASN52 ARG56 THR57 ASN58 ASP61 LYS64 ASN96 ASN98 LEU99 THR101 GLY102 THR103 LEU104 SER105 GLY106 ALA107 ARG108 LEU109 TYR110 TRP111 D: GLN35 SER36 GLY37 PHE38 VAL39 TYR40 GLU41 VAL44 GLN46 ARG73 PRO103 GLN104 LEU105 GLU106 PRO107 SER108 CYS109 ASN110 LYS111 HIS112 PHE113 PHE115 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
