Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5582 |
| PDBID: | 7X8T |
| Chains: | IH_G |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.51 |
| Reference: | 10.1016/j.str.2022.11.009 |
| Antibody | |
| Antibody: | hB9L9.3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human frizzled-10 cysteine-rich domain (CRD) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >7X8T_I|Chain C, F, I, L|Antibody hB9L9.3 Fab, Heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSFNMFWVRQAPGKGLEWVAGIDDDGSYPNYGSAVKGRATISRDNSKNTLYLQMNSLRAEDTAVYYCAKSGYGGSWGGYIADDIDAWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHHHH |
Light Chain: H
Mutation: NULL
| >7X8T_H|Chain B, E, H, K|Antibody hB9L9.3 Fab, Light chain|Homo sapiens (9606) ELTQPPSVSVSPGQTARITCSGDGSYAGSYYYGWYQQKPGQAPVTLIYYNNKRPSGIPERFSGSLSGSTNTLTISGVQAEDEADYYCGSADNSGAAFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: G
Mutation: N25Q
| >7X8T_G|Chain A, D, G, J|Frizzled-10|Homo sapiens (9606) MDMERPGDGKCQPIEIPMCKDIGYQMTRMPNLMGHENQREAAIQLHEFAPLVEYGCHGHLRFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFNFKWPDSLDCRKLPNKNDPNYLCMEAPN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR26 ALA27 GLY28 SER29 TYR31 TYR49 ALA90 ASP91 ASN92 SER93 GLY94 I: TYR57 PRO58 ASN59 TYR60 LYS65 TYR101 GLY102 GLY103 SER104 TRP105 GLY106 GLY107 TYR108 ILE109 ASP111 ASP112 G: LEU55 MET56 LEU90 PRO104 ARG107 VAL108 GLU111 GLN112 ARG114 LEU115 SER131 LEU132 ASP133 ARG135 LYS136 LEU137 PRO138 ASN139 LYS140 ASN141 ASP142 PRO143 ASN144 TYR145 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
