Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5578 |
| PDBID: | 7X8Q |
| Chains: | CB_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.65 |
| Reference: | 10.1016/j.str.2022.11.009 |
| Antibody | |
| Antibody: | F10_A9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Frizzled-10 cysteine-rich domain (CRD) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >7X8Q_C|Chain C, F|Antibody F10_A9 Fab, Heavy chain|synthetic construct (32630) EVQLVESGGGLVQPGGSLRLSCAASGFPIRGSSIHWVRQAPGKGLEWVAATYGWPGSITYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARRHTYPLWALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCHHHHHHHH |
Light Chain: B
Mutation: NULL
| >7X8Q_B|Chain B, E|Antibody F10_A9 Fab, Light chain|synthetic construct (32630) DIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQGWPFFTFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: N25Q
| >7X8Q_A|Chain A, D|Frizzled-10|Homo sapiens (9606) MDMERPGDGKCQPIEIPMCKDIGYQMTRMPNLMGHENQREAAIQLHEFAPLVEYGCHGHLRFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFNFKWPDSLDCRKLPNKNDPNYLCMEAPN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: SER28 SER30 ALA32 TRP92 PRO93 PHE94 C: PRO28 ARG30 GLY31 SER33 TYR52 GLY53 TRP54 ARG99 THR101 TYR102 TRP105 A: PRO40 MET41 LYS43 ASP44 ARG62 ILE66 HIS69 GLU70 ALA72 PRO73 LEU74 VAL75 GLU76 TYR77 ARG84 ILE120 PHE124 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
