Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5577 |
| PDBID: | 7X8P |
| Chains: | FE_D |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.24 |
| Reference: | 10.1016/j.str.2022.11.009 |
| Antibody | |
| Antibody: | pF7_A5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human frizzled-2 cysteine-rich domain (CRD) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >7X8P_F|Chain C, F|Antibody pF7_A5 Fab, Heavy chain|synthetic construct (32630) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSFNMFWVRQAPGKGLEWVAGIDDDGSYPNYGSAVKGRATISRDNSKNTLYLQMNSLRAEDTAVYYCAKSGGWALGGLITLRWIDAWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: E
Mutation: NULL
| >7X8P_E|Chain B, E|Antibody pF7_A5 Fab, Light chain|synthetic construct (32630) ELTQPPSVSVSPGQTARITCSGDGSIYRVFSYGWYQQKPGQAPVTLIYLNNKRPSGIPERFSGSLSGSTNTLTISGVQAEDEADYYCGSFGNAPLAFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: D
Mutation: NULL
| >7X8P_D|Chain A, D|Frizzled-2|Homo sapiens (9606) PDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPERLRCEHFPRHGAEQICVGQNHSEDGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition E: ILE26 TYR27 ARG28 VAL29 PHE30 SER31 LEU49 ASN50 LYS52 LEU65 GLY91 ASN92 ALA93 PRO94 LEU95 F: TRP47 TYR57 ASN59 TYR60 TRP102 LEU104 GLY105 GLY106 LEU107 ILE108 LEU110 D: ALA51 TYR52 LEU60 PHE90 MET95 VAL99 CYS100 THR101 VAL102 LEU103 GLN105 ALA106 ILE107 PRO108 ARG111 ARG135 GLU139 HIS140 PHE141 PRO142 HIS144 GLY145 ALA146 GLU147 GLN148 ILE149 CYS150 VAL151 GLY152 GLN153 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
