Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5575 |
| PDBID: | 7X7V |
| Chains: | AF_E |
| Organism: | Mus musculus, Severe acute respiratory syndrome-related coronavirus |
| Method: | EM |
| Resolution (Å): | 3.83 |
| Reference: | 10.1073/pnas.2204256119 |
| Antibody | |
| Antibody: | X17 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7X7V_A|Chain D[auth A]|X17 heavy chain|Mus musculus (10090) QVQLQQSGAELARPGASVKLSCKASGYTFTFYWMQWLKQRPGQGLEWIGAIYPGDGDTRYTQRFKDKATLTADKSSSTAYIQLSSLASEDSAVYYCAGGEYDNYGFDYWGQGTTLTVSS |
Light Chain: F
Mutation: NULL
| >7X7V_F|Chain C[auth F]|X17 light chain|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCRASQDISNYLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGTTLPYTFGGGTKLEIK |
Antigen
Chain: E
Mutation: NULL
| >7X7V_E|Chain G[auth E]|Spike protein S1|Severe acute respiratory syndrome coronavirus (694009) TNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition F: TYR32 TYR49 TYR50 LEU54 SER56 GLY91 THR92 THR93 LEU94 TYR96 A: TYR27 THR28 PHE31 TYR32 TRP33 TYR52 ASP55 ASP57 ARG59 GLU100 TYR101 ASP102 ASN103 TYR104 ASP107 TYR108 E: TRP340 GLU341 ARG342 LYS344 SER380 ASN381 TYR383 PRO413 ASP414 ASP415 PHE416 ARG449 PRO450 PHE451 GLU452 ARG453 ILE455 GLU502 LEU504 ASN505 ALA506 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
