Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5574 |
| PDBID: | 7X7V |
| Chains: | HL_E |
| Organism: | Mus musculus, Severe acute respiratory syndrome-related coronavirus |
| Method: | EM |
| Resolution (Å): | 3.83 |
| Reference: | 10.1073/pnas.2204256119 |
| Antibody | |
| Antibody: | X10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7X7V_H|Chain B[auth H]|X10 heavy chain|Mus musculus (10090) EVQLQQSGPELVKPGASVKISCKTSGYTFTEYTLHWVKQSHGKSLEWIGGFDPNFGGATYNLKFEDKATLTVDKSSNTAYMELRSLTSEDSAVFYCARGDYGTSYAYFDFWGQGTTLTVSS |
Light Chain: L
Mutation: NULL
| >7X7V_L|Chain A[auth L]|X10 light chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRAAISCRASQSVSTSSHNYVHWYQQRPGQPPKLLIKYASNLECGVPARFSGSGSGTDFTLNIHPVEEEDSAAYYCQHSWEIPYTFGGGTKLEIK |
Antigen
Chain: E
Mutation: NULL
| >7X7V_E|Chain G[auth E]|Spike protein S1|Severe acute respiratory syndrome coronavirus (694009) TNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition L: GLN27 SER28 SER30 THR31 SER32 SER33 HIS34 TYR36 TYR54 SER71 GLY72 SER95 TRP96 GLU97 ILE98 TYR100 H: GLU31 ASP52 ASN54 PHE55 GLY57 ALA58 THR59 TYR101 GLY102 THR103 SER104 TYR105 E: ASN330 ALA331 THR332 LYS333 TYR338 GLU341 TRP423 ASN427 ILE428 ASP429 ALA430 THR431 SER432 GLY434 ASN435 TYR436 ASN437 TYR438 LYS439 TYR442 ILE455 SER456 ASN457 THR468 PRO469 PRO470 TYR475 TRP476 LEU478 ASN479 ASP480 ARG495 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
