Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5549 |
| PDBID: | 7X6L |
| Chains: | HL_F |
| Organism: | Homo sapiens, Influenza A virus (A/Hong Kong/1/1968(H3N2)), Influenza A virus |
| Method: | EM |
| Resolution (Å): | 3.70 |
| Reference: | 10.1038/s41467-022-29950-w |
| Antibody | |
| Antibody: | 28-12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus HongKong/01/1968 H3N2 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | P03437 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7X6L_H|Chain A[auth G], E[auth H], I|Heavy chain of antibody 12 fab|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFSTYNMNWVRQAPGKGLEWLSYISTSSNTIYYADSVKGRFTISRDNAKNSLFLQMNSLRDEDTAVYYCARDRGCSSTNCYVVGYYFYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVE |
Light Chain: L
Mutation: NULL
| >7X6L_L|Chain B[auth J], F[auth K], J[auth L]|The light chain of the antibody 12 fab|Homo sapiens (9606) DIQMTQSPSSVSASVGDRVTITCRASQGISSYLAWYQLKPGRAPKLLIYGATRLQSGVPSRFSGSGSGTDFTLTISGLQPEDFATYHCQQADSFPLTFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: F
Mutation: NULL
| >7X6L_F|Chain D[auth B], H[auth E], L[auth F]|Hemagglutinin|Influenza A virus (11320) GLFGAIAGFIENGWEGMIDGWYGFRHQNSEGTGQAADLKSTQAAIDQINGKLNRVIEKTNEKFHQIEKEFSEVEGRIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFEKTRRQLRENAEDMGNGCFKIYHKCDNACIESIRNGTYDHDVYRDEALNNRFQIKGV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER52 SER54 ASN56 THR57 TYR59 GLY101 CYS102 SER103 SER104 THR105 ASN106 CYS107 TYR108 VAL109 VAL110 GLY111 TYR112 TYR113 PHE114 TYR115 L: TYR32 ASP92 SER93 PHE94 F: ILE18 ASP19 GLY20 TRP21 ALA36 LEU38 LYS39 THR41 GLN42 ALA43 ILE45 ASP46 ILE48 ASN49 GLY50 LEU52 ASN53 ILE56 GLU57 LYS58 GLU150 ARG153 ASN154 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
