Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5536 |
| PDBID: | 7X47 |
| Chains: | HL_B |
| Organism: | Mus musculus, Coxsackievirus B1 |
| Method: | EM |
| Resolution (Å): | 3.66 |
| Reference: | 10.1016/j.chom.2022.08.001 |
| Antibody | |
| Antibody: | 2E6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Coxsackievirus B1 empty particle VP2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7X47_H|Chain B[auth H]|2E6 heavy chain|Mus musculus (10090) QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWRGGSTDYNAAFMSRLSITKDNSKSQVFFKMNSLQADDTAIYYCAKGDYYGYDAMDSWGQGTSVTVSR |
Light Chain: L
Mutation: NULL
| >7X47_L|Chain A[auth L]|2E6 light chain|Mus musculus (10090) DIQMTQSPASLSVSVGETVTITCRASENVYRNLAWYQQKQGKSPQLLVYAATNLADGVPSRFSGSGSGTQYSLKINSLQSEDFGSYFCQHFWSPVFTFGAGTKLELK |
Antigen
Chain: B
Mutation: NULL
| >7X47_B|Chain D[auth B]|VP2|Coxsackievirus B1 (12071) SPSAEECGYSDRVRSITLGNSTITTQECANVVVGYGVWPEYLKDNEATAEDQPTQPDVATCRFYTLESVQWMKNSAGWWWKLPDALSQMGLFGQNMQYHYLGRTGYTIHVQCNASKFHQGCLLVVCVPEAEMGCSNLNNTPEFSELSGGDSARMFTDTQVGESNAKKVQTAVWNAGMGVGVGNLTIFPHQWINLRTNNSATLVMPYINSVPMDNMFRHNNLTLMIIPFVPLNYSEGSSPYVPITVTIAPMCAEYNGLRLASNQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR30 ARG31 ASN32 TYR49 ALA50 ASN53 LEU54 ASP56 SER60 PHE91 TRP92 SER93 PRO94 PHE96 H: THR30 ASN31 TRP52 ARG53 GLY54 SER56 ASP58 TYR101 GLY102 TYR103 ASP104 B: GLN70 MET72 ASN74 ALA76 GLU142 SER144 GLU145 GLY148 GLY149 SER151 ARG153 MET154 PHE155 THR156 ASP157 THR158 GLN159 VAL160 GLY161 GLU162 SER163 ASN164 ALA165 LYS167 THR170 GLU235 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
