Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5534 |
| PDBID: | 7X42 |
| Chains: | HL_C |
| Organism: | Mus musculus, Coxsackievirus B1 |
| Method: | EM |
| Resolution (Å): | 3.88 |
| Reference: | 10.1016/j.chom.2022.08.001 |
| Antibody | |
| Antibody: | 8A10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Coxsackievirus B1 pre-A particle VP3 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7X42_H|Chain B[auth H]|8A10 heavy chain|Mus musculus (10090) QVQLQQSAAELARPGASVKMSCKASGYTFTTYTMHWVKQRPGQGLEWIGYINPSSRYTEYNQKFKDKTTLTADKSSSTAYMQLSSLTFEDSAVYYCARRSEADRFVYWGQGTLVTVSA |
Light Chain: L
Mutation: NULL
| >7X42_L|Chain A[auth L]|8A10 light chain|Mus musculus (10090) DIQMTQTKSSLSASLGDRVTISCRASQDISNYLNWYQQKPDGSVKLLIYYTSTLHSGVPSRFSGSGSGTDYSLTINSLEQEDIATYFCQQGNTFPFTFGGGTKLEIRR |
Antigen
Chain: C
Mutation: NULL
| >7X42_C|Chain E[auth C]|VP3|Coxsackievirus B1 (12071) GLPVMTTPGSTQFLTSDDFQSPSAMPQFDVTPEMQIPGRVNNLMEIAEVDSVVPVNNTEDNVSSLKAYQIPVQSNSDNGKQVFGFPLQPGANNVLNRTLLGEILNYYTHWSGSIKLTFMFCGSAMATGKFLLAYSPPGAGVPKNRKDAMLGTHVIWDVGLQSSCVLCVPWISQTHYRYVVEDEYTAAGYVTCWYQTNIVVPADVQSSCDILCFVSACNDFSVRMLKDTPFIRQDTFYQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR32 LEU46 TYR49 TYR50 THR53 HIS55 H: GLY26 TYR27 THR28 TYR32 SER54 SER55 ARG56 LYS74 ARG98 SER100 GLU101 ALA102 ARG104 VAL106 TYR107 C: GLU59 ASP60 VAL62 SER63 ARG232 GLN233 ASP234 THR235 PHE236 TYR237 GLN238 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
