Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 549 |
| PDBID: | 3JBQ |
| Chains: | hl_B |
| Organism: | Mus musculus, Bos taurus |
| Method: | EM |
| Resolution (Å): | 11.00 |
| Reference: | 10.1074/jbc.M115.647636 |
| Antibody | |
| Antibody: | 2E8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | phosphodiesterase 5/6 chimera catalytic domain |
| Antigen mutation: | No |
| Durg Target: | O76074 |
Sequence information
Antibody
Heavy Chain: h
Mutation: NULL
| >3JBQ_h|Chain B[auth H], J[auth h]|IgG1-kappa 2E8 heavy chain|Mus musculus (10090) EVQLQQSGAEVVRSGASVKLSCTASGFNIKDYYIHWVKQRPEKGLEWIGWIDEIGDTEYVPKFQGKATMTADTSSNTAYLQLSSLTSEDTAVYYCNAGHDYDRGRFPYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: l
Mutation: NULL
| >3JBQ_l|Chain A[auth L], I[auth l]|IgG1-kappa 2E8 light chain|Mus musculus (10090) DIVMTQSQKFMSTSVGDRVSITCKASQNVGTAVAWYQQKPGQSPKLMIYSASNRYTGVPDRFTGSGSGTDFTLTISNMQSEDLADYFCQQYSSYPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSATDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >3JBQ_B|Chain C[auth B], F|phosphodiesterase 5/6 chimera catalytic domain|Bos taurus (9913) GSHMEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFWEQGDLERTVLQQQPIPMMDRNKRDELPKLQVGFIDFVCTQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| l: TYR94 h: LYS30 ASP31 TYR33 TRP50 ASP52 GLU53 ILE54 ASP56 GLU58 THR73 ASP97 TYR98 B: THR784 GLU788 ASP791 ARG794 THR795 VAL796 GLN800 PRO801 ASP806 ARG807 ASN808 ARG810 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
