Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5487 |
| PDBID: | 7WVL |
| Chains: | HL_F |
| Organism: | Mus musculus, Severe acute respiratory syndrome coronavirus 2 |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1371/journal.ppat.1010994 |
| Antibody | |
| Antibody: | P4A2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7WVL_H|Chain B[auth H]|P4A2 Fab heavy chain|Mus musculus (10090) QVQLQQSGAELVKPGASVKLSCKASGFTFTRYSIYWMKQRPGQGLEWIGEINPSTGDTNFNEKFKSKATLTVDKSSTTAYMQLSSLTSEDSAVYYCTRSTGYWGQGTLVTVSAAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP |
Light Chain: L
Mutation: NULL
| >7WVL_L|Chain A[auth L]|P4A2 Fab Light Chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASESVEYHGTILMQWFQQKPGQPPRLLIYAASNVDSGVPARFSGSGSGTDLSLNIHPVEEDDIAMYFCQQSRKVPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKINGSQRQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCQATHKTSTSPIVKSFNRNAC |
Antigen
Chain: F
Mutation: NULL
| >7WVL_F|Chain C[auth F]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) ITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNGSLEVLFQGPGSHHHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR31 HIS32 THR34 LEU36 GLN38 PHE40 TYR53 ALA54 ASN57 VAL58 ASP59 SER60 ALA64 GLN93 SER95 ARG96 TYR100 H: THR30 ARG31 TYR32 SER33 TYR35 MET37 GLU50 ASN52 SER54 THR97 SER99 F: LYS417 LEU455 PHE456 TYR473 GLN474 ALA475 GLY476 SER477 THR478 CYS480 ASN481 GLY482 VAL483 GLU484 GLY485 PHE486 ASN487 TYR489 PHE490 GLN493 TYR505 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
