Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5484 |
| PDBID: | 7WUR |
| Chains: | EF_A |
| Organism: | dengue virus type 2, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1038/s41467-022-34415-1 |
| Antibody | |
| Antibody: | 5E3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dengue virus type 2 secreted NS1 (sNS1) |
| Antigen mutation: | No |
| Durg Target: | P12823; P27914; |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7WUR_E|Chain C[auth E], E[auth G]|Fab 5E3 Heavy Chain|Homo sapiens (9606) QVQLVESGGGLVKPGGSLRLSCAASGFTFSDYYMSWIRQAPGKGLEWVSYISTGSSHTDYADSVKGRFTISRDNTKNSLYLQLNSLRDEDTAVYYCASGYRFSYVSYWGQGTLVTVSS |
Light Chain: F
Mutation: NULL
| >7WUR_F|Chain D[auth F], F[auth H]|Fab 5E3 Light Chain|Homo sapiens (9606) EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASTRATGIPDRFTGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPAWTFGQGTKVEI |
Antigen
Chain: A
Mutation: NULL
| >7WUR_A|Chain A, B|Core protein|Dengue virus 2 (11060) DSGCVVSWKNKELKCGSGIFITDNVHTWTEQYKFQPESPSKLASAIQKAHEEGICGIRSVTRLENLMWKQITPELNHILTENEVKLTIMTGDIKGIMQAGKRSLRPQPTELKYSWKAWGKAKMLSTELHNHTFLIDGPETAECPNTNRAWNSLEVEDYGFGVFTTNIWLKLKERQDVFCDSKLMSAAIKDNRAVHADMGYWIESALNDTWKIEKASFIEVKSCHWPKSHTLWSNGVLESEMIIPKNFAGPVSQHNYRPGYHTQTAGPWHLGRLEMDFDFCEGTTVVVTEDCGNRGPSLRTTTASGKLITEWCCRSCTLPPLRYRGEDGCWYGMEIRPLKEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ASP31 TYR32 TYR33 SER52 THR53 GLY54 SER56 HIS57 SER98 GLY99 TYR100 ARG101 PHE102 SER103 TYR104 SER106 F: GLU1 ILE2 GLN27 SER28 TYR33 TYR92 SER94 A: ASP92 LYS94 PRO138 HIS269 GLU281 GLY282 ARG299 THR301 THR302 ALA303 SER304 GLY305 LYS306 LEU307 THR309 ARG324 GLY325 GLU326 ASP327 GLY328 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
