Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5479 |
| PDBID: | 7WUE |
| Chains: | EF_B |
| Organism: | Severe acute respiratory syndrome coronavirus 2, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1126/scitranslmed.abm0899 |
| Antibody | |
| Antibody: | m31A7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein S1 |
| Antigen mutation: | No |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7WUE_E|Chain B[auth C], E|m31A7 Fab HEAVY CHAIN|Mus musculus (10090) MGWSLILLFLVAVATRVEVQLQQSGPEMVKPGASVKISCKTSGYTFTEYTIYWVKQSHGKSLEWLGGINPNIGDTTYNQKFKGKATLTVDTSSSTAYMELRSLTSEDSAVYYCAREVYNYSFAYWGQGTLVTVSAASTTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKAEPKSC |
Light Chain: F
Mutation: NULL
| >7WUE_F|Chain C[auth D], F|m31A7 Fab LIGHT CHAIN|Mus musculus (10090) MRVPAQLLGLLLLWLPGARCDIVMSQSPSSLAVSVGEKVTMSCKSSQSLLYSSNQKNYLAWYQQKLGQTPKLLIYWASSRESGVPDRFTGSGSGTDFTLTISSVRAEDLAVYYCQQYYRYPLTFGVGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7WUE_B|Chain A, D[auth B]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) TNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: GLU31 TYR32 THR33 TYR35 GLY50 ILE51 ASN52 ASN54 ILE55 ASP57 THR58 THR59 GLU99 TYR101 TYR103 SER104 F: TYR31 SER32 ASN34 TYR38 TYR97 TYR98 ARG99 TYR100 LEU102 B: PHE456 LYS458 GLN474 ALA475 GLY476 SER477 THR478 PRO479 CYS480 ASN481 PHE486 ASN487 CYS488 TYR489 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
