Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5368 |
| PDBID: | 7WN8 |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1186/s13045-023-01399-4 |
| Antibody | |
| Antibody: | BC31M5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human leukocyte surface antigen CD47 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7WN8_H|Chain C[auth H], E[auth P]|BC31M5 Fab Heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYGISWVRQAPGQGLEWMGWISAYNGNTNYAQKLQGRVTMTTDTSTSTAYMELRSLRSDDTAVYYCARGTVYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKAEPKSCDKTHTHHHHHH |
Light Chain: L
Mutation: NULL
| >7WN8_L|Chain D[auth L], F[auth Q]|BC31M5 Fab Light chain|Homo sapiens (9606) EIVLTQSPATLSLSPGERATLSCRASQSLSRNHLAWYQQKPGQPPRLLIHWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQHTQNTWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: C15G
| >7WN8_A|Chain A, B[auth C]|Leukocyte surface antigen CD47|Homo sapiens (9606) MWPLVAALLLGSACGGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 SER31 TYR32 GLY33 TRP50 SER52 TYR54 ARG98 GLY99 THR100 VAL101 TYR102 ASP104 L: SER30 ARG31 ASN32 HIS33 HIS50 TRP51 SER53 THR54 HIS92 GLN94 ASN95 A: GLN19 LEU21 GLU47 THR52 GLU53 VAL54 TYR55 LYS57 ASP64 ASP69 ALA71 LEU72 GLU115 THR117 LEU119 THR120 ARG121 GLU122 GLU124 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
