Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5365 |
| PDBID: | 7WN1 |
| Chains: | C_A |
| Organism: | Plasmodium falciparum, Vicugna pacos |
| Method: | EM |
| Resolution (Å): | 3.11 |
| Reference: | 10.1038/s41467-023-37411-1 |
| Antibody | |
| Antibody: | 48 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum nucleoside transporter ENT1 (PfENT1) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >7WN1_C|Chain B[auth C]|nanobody48|Vicugna pacos (30538) QVQLVESGGGLVQPGGSLRLSCAASGFTGSINYMGWYRQAPGKQRELVARFSSGGSTNYADSVKGRFTISGDNAKNTVYLQMNSLKPEDTAVYYCNAETISYVYTVVFQDYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: Y190A
| >7WN1_A|Chain A|Equilibrative nucleoside/nucleobase transporter|Plasmodium falciparum (5833) MSTGKESSKAYADIESRGDYKDDGKKGSTLSSKQHFMLSLTFILIGLSSLNVWNTALGLNINFKYNTFQITGLVCSSIVALFVEIPKIMLPFLLGGLSILCAGFQISHSFFTDTQFDTYCLVAFIVIGVVAGLAQTIAFNIGSTMEDNMGGYMSAGIGISGVFIFVINLLLDQFVSPEKHYGVNKAKLLALYIICELCLILAIVFCVCNLDLTNKNNKKDEENKENNATLSYMELFKDSYKAILTMFLVNWLTLQLFPGVGHKKWQESHNISDYNVTIIVGMFQVFDFLSRYPPNLTHIKIFKNFTFSLNKLLVANSLRLLFIPWFILNACVDHPFFKNIVQQCVCMAMLAFTNGWFNTVPFLVFVKELKKAKKKKEIEIISTFLVIAMFVGLFCGIWTTYIYNLFNIVLPKPDLPPIDVTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: SER25 GLY26 PHE27 THR28 GLY29 SER30 ASN32 TYR33 TYR37 ARG50 SER52 SER53 GLY54 SER56 THR57 ASN58 ASN73 ALA74 GLU98 ILE100 SER101 TYR102 VAL103 TYR104 THR105 VAL106 VAL107 PHE108 GLN109 ASP110 A: PHE139 SER143 GLU146 ASP147 ASN148 GLY150 GLY151 SER154 ILE157 GLY158 MET246 ASP287 PHE288 ARG291 TYR292 ASN295 LEU296 THR297 HIS298 LYS303 ASN358 THR359 PHE362 LEU363 PHE365 VAL366 LYS367 LEU369 LYS370 ALA372 LYS373 LYS374 LYS375 ILE378 GLU379 SER382 VAL386 MET389 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
