Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5364 |
| PDBID: | 7WN0 |
| Chains: | B_A |
| Organism: | Plasmodium falciparum, Vicugna pacos |
| Method: | EM |
| Resolution (Å): | 3.64 |
| Reference: | 10.1038/s41467-023-37411-1 |
| Antibody | |
| Antibody: | 19 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum nucleoside transporter ENT1 (PfENT1) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7WN0_B|Chain B|nanobody19|Vicugna pacos (30538) QLQLVESGGGLVQAGGSLRLSCAASGSTSNINVMGWYRQAPGKQRELVATISSGDALNYANSVEGRFTISRDAAKNTVYLQMNSLKPEDSAVYICNAYVVSSYGYRASWNDYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: Y190A
| >7WN0_A|Chain A|Equilibrative nucleoside/nucleobase transporter|Plasmodium falciparum (5833) MSTGKESSKAYADIESRGDYKDDGKKGSTLSSKQHFMLSLTFILIGLSSLNVWNTALGLNINFKYNTFQITGLVCSSIVALFVEIPKIMLPFLLGGLSILCAGFQISHSFFTDTQFDTYCLVAFIVIGVVAGLAQTIAFNIGSTMEDNMGGYMSAGIGISGVFIFVINLLLDQFVSPEKHYGVNKAKLLALYIICELCLILAIVFCVCNLDLTNKNNKKDEENKENNATLSYMELFKDSYKAILTMFLVNWLTLQLFPGVGHKKWQESHNISDYNVTIIVGMFQVFDFLSRYPPNLTHIKIFKNFTFSLNKLLVANSLRLLFIPWFILNACVDHPFFKNIVQQCVCMAMLAFTNGWFNTVPFLVFVKELKKAKKKKEIEIISTFLVIAMFVGLFCGIWTTYIYNLFNIVLPKPDLPPIDVTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: THR28 SER29 ASN30 ASN32 VAL33 TYR37 LEU47 THR50 SER52 SER53 GLY54 ASP55 ALA56 LEU57 ASN58 ASN96 TYR98 VAL100 SER101 SER102 TYR103 GLY104 TYR105 ARG106 ALA107 SER108 TRP109 ASN110 ASP111 A: SER77 LEU81 GLN135 THR136 PHE139 ASN140 SER143 ASN148 GLY150 GLY151 SER154 ALA155 ILE157 GLY158 ARG291 TYR292 ASN295 LEU296 THR297 HIS298 ASN358 THR359 PHE362 LEU363 PHE365 VAL366 LYS367 LEU369 LYS370 ALA372 LYS373 LYS374 LYS375 ILE378 GLU379 SER382 VAL386 MET389 PHE390 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
