Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5355 |
| PDBID: | 7WKI |
| Chains: | B_A |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1016/j.jbc.2022.101962 |
| Antibody | |
| Antibody: | Anti-CFH Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human complement factor H |
| Antigen mutation: | No |
| Durg Target: | P08603 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7WKI_B|Chain B|Anti-CFH nanobody (VHH)|Vicugna pacos (30538) QVQLVESGGGLVQAGGSLRLSCAASGLTVDDYAIGWFRQAPGKEREGVSCISSSNGSTYYADSVKGRFTISSDNAKNTAYLQMNSLKPEDTAVYYCAAAVSPNLECGTGPFGIYASYYGMDYWGQGTQVTVSSAAGHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7WKI_A|Chain A|Complement factor H|Homo sapiens (9606) TSCVNPPTVQNAYIVSRQMSKYPSGERVRYQCRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYSRTGESVEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKRHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ALA33 GLN39 ARG45 CYS50 TYR95 ALA99 VAL100 SER101 PRO102 ASN103 LEU104 GLU105 CYS106 THR108 GLY109 PRO110 PHE111 TYR114 SER116 TYR117 TYR118 GLY119 MET120 ASP121 TRP123 GLY124 GLN125 A: ILE1169 ARG1171 MET1174 ALA1180 LEU1181 ARG1182 TRP1183 THR1184 ALA1185 GLN1187 LYS1188 LEU1189 VAL1200 CYS1201 LYS1202 ARG1203 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
