Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 534 |
| PDBID: | 3J8W |
| Chains: | IM_B |
| Organism: | Mus musculus, Human papillomavirus 16 |
| Method: | EM |
| Resolution (Å): | 13.00 |
| Reference: | 10.1016/j.virol.2015.04.016 |
| Antibody | |
| Antibody: | H263.A2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Major capsid protein L1 from Human papillomavirus type 16 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >3J8W_I|Chain B[auth F], D[auth G], F[auth H], H[auth I]|H263.A2 heavy chain|Mus musculus (10090) LQQSGAELARPGASVKLSCKASGYTFISYWMQWVKQRPGQGLEWIGAIYPGDGATRYTQKFKGKATLTADKSSSTAYMQLSSLTSEDSAVYYCARPSYYDYDVTWFAYWGQGTLVTVS |
Light Chain: M
Mutation: NULL
| >3J8W_M|Chain A[auth J], C[auth K], E[auth L], G[auth M]|H263.A2 light chain|Mus musculus (10090) DIVMTQTPASLSASVGETVTITCRASENIYSYLAWYQQKQGKSPQLLVYNAETLAEGVPSRFSGSGSGTQFSLRINSLQPEDFGSYYCQHHYVSPLTFGAGTKLELKRA |
Antigen
Chain: B
Mutation: NULL
| >3J8W_B|Chain I[auth A], J[auth B], K[auth C], L[auth D], M[auth E]|L1|Human papillomavirus type 16 (333760) AVVSTDEYVARTNIYYHAGTSRLLAVGHPYFPIKKPNNNKILVPKVSGLQYRVFRIHLPDPNKFGFPDTSFYNPDTQRLVWACVGVEVGRGQPLGVGISGHPLLNKLDDTENASAYAANAGVDNRECISMDYKQTQLCLIGCKPPIGEHWGKGSPCTQVAVQPGDCPPLELINTVIQDGDMVDTGFGAMDFTTLQANKSEVPLDICTSICKYPDYIKMVSEPYGDSLFFYLRREQMFVRHLFNRAGTVGENVPDDLYIKGSGSTANLASSNYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMSLCAAISTSETTYKNTNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNSTILEDWNFGLQPPPGGTLEDTYRFVTSQAIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSADLDQFPLGRKFLLQLGL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: TYR30 SER31 TYR32 HIS91 TYR92 VAL93 SER94 I: THR28 ILE30 SER31 TRP33 ASP54 ARG58 LYS73 SER74 TYR97 TYR98 ASP99 B: LEU126 TYR135 ALA136 ALA137 ASN138 ALA139 GLY140 VAL178 GLN181 PRO182 GLY183 ASP184 GLY265 THR266 VAL267 GLY268 ASN270 ASP273 LYS278 GLY279 SER280 GLY281 SER282 THR283 ALA284 ASN285 LEU286 ALA287 SER288 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
