Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5301 |
| PDBID: | 7W71 |
| Chains: | HL_A |
| Organism: | Escherichia coli, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1126/sciadv.abp9011 |
| Antibody | |
| Antibody: | 12C7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Escherichia coli regulator of sigma-E protease RseP- C domain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7W71_H|Chain C[auth H], E[auth I]|Heavy chain of Fab|Mus musculus (10090) QVQLQQSRAELARPGASVKMSCKASGYTFTTYTMQWVKQRPGQALEWIGYINPGSGYAKNNQKFKDKATLTADKSSSTAYMQLSSLTSDDSAVYYCARSGSFFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >7W71_L|Chain D[auth L], F[auth M]|Light chain of Fab|Mus musculus (10090) DIVLTQSPAILSVTPGDSVSLSCRASQSVSSNLHWYQQRSHESPRLLITYAFQSISGIPSRFSGNGSGTDFTLNINSVETEDFGMYFCQQSNSWPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >7W71_A|Chain A, B|Regulator of sigma-E protease RseP|Escherichia coli (562) GSGPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEIERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 THR30 THR31 TYR32 THR33 TYR50 ASN52 PRO53 SER55 TYR57 LYS59 SER99 GLY100 SER101 PHE102 L: ASN32 THR49 TYR50 PHE52 GLN53 SER54 ILE55 SER91 ASN92 SER93 TRP94 TYR96 A: VAL229 PRO231 ASN232 SER236 LYS237 GLY239 LEU240 GLN241 ALA242 SER271 ALA273 GLU275 ARG278 GLN279 SER281 PRO282 LEU283 SER284 LEU285 THR286 ILE288 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
