Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5283 |
| PDBID: | 7VBH |
| Chains: | N_A |
| Organism: | Homo sapiens, Escherichia coli, Rattus norvegicus, Bos taurus |
| Method: | EM |
| Resolution (Å): | 3.00 |
| Reference: | 10.1038/s41467-022-28683-0 |
| Antibody | |
| Antibody: | 35 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short |
| Antigen mutation: | No |
| Durg Target: | P63092 |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7VBH_N|Chain B[auth N]|Nanobody 35|Homo sapiens (9606) MAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7VBH_A|Chain A|Guanine nucleotide-binding protein G(s) subunit alpha isoforms short|Homo sapiens (9606) MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: LYS33 LYS43 GLY44 LEU45 GLU46 TRP47 ASP50 SER59 TYR60 THR61 GLY62 SER63 LYS65 PRO100 THR104 ARG105 ASP106 CYS107 PHE108 ASP109 THR111 SER112 THR113 THR114 TYR115 ALA116 TYR117 A: ARG228 ASP229 GLU230 ARG231 ARG232 ILE235 THR263 ASN264 GLN267 ALA268 LYS271 LEU272 ASP274 SER275 ILE276 ASN278 ASN279 LYS280 ARG283 ASP310 TYR311 PHE312 PRO313 GLU314 ALA351 SER352 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
