Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5282 |
| PDBID: | 7VAG |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.32 |
| Reference: | 10.1038/s41586-022-04845-4 |
| Antibody | |
| Antibody: | YN69202 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | human NTCP-myr-preS1 |
| Antigen mutation: | Yes |
| Durg Target: | Q14973 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7VAG_H|Chain B[auth H]|Fab heavy chain from antibody IgG clone number YN69202|Mus musculus (10090) EVQLQQSGPELVKPGASVKMSCKASGYTFTSYIIHWVKQKPGQGLEWIGYINPYNDGTKYNEKFKGKGTLTSDKSSSTAYMELSSLTSEDSAVYYCARSYYDGIPHYFDYWGQGTTLTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPSGPISTINPCPPCKECHKCPAPNLEGGPS |
Light Chain: L
Mutation: NULL
| >7VAG_L|Chain C[auth L]|Fab light chain from antibody IgG clone number YN69202|Mus musculus (10090) DIVMTQSPSSLAVSAGEKVTMSCKSSQSLFNSRTRRNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCKQSYYLLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: Q261A
| >7VAG_A|Chain A|Sodium/bile acid cotransporter|Homo sapiens (9606) MEAHNASAPFNFTLPPNFGKRPTDLALSVILVFMLFFIMLSLGCTMEFSKIKAHLWKPKGLAIALVAQYGIMPLTAFVLGKVFRLKNIEALAILVCGCSPGGNLSNVFSLAMKGDMNLSIVMTTCSTFCALGMMPLLLYIYSRGIYDGDLKDKVPYKGIVISLVLVLIPCTIGIVLKSKRPQYMRYVIKGGMIIILLCSVAVTVLSAINVGKSIMFAMTPLLIATSSLMPFIGFLLGYVLSALFCLNGRCRRTVSMETGCANVQLCSTILNVAFPPEVIGPLFFFPLLYMIFQLGEGLLLIAIFWCYEKFKTPKDKTKMIENLYFQGDYKDDDDKHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 THR30 SER31 TYR32 TYR50 ASN52 TYR54 ASN55 LYS59 TYR100 TYR101 ASP102 GLY103 ILE104 PRO105 HIS106 TYR107 L: ASN31 SER32 ARG33 THR34 ARG35 ARG36 TYR38 TYR55 TRP56 THR59 GLU61 TYR99 A: ASN5 ALA6 SER7 ALA8 PRO9 PHE10 PHE77 GLY80 LYS81 ARG84 LEU85 LYS86 ASN87 ILE88 ALA90 GLY144 ILE145 TYR146 ASP147 GLY148 LYS153 ASN271 VAL272 ALA273 PHE274 PRO275 PRO276 GLU277 VAL278 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
