Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5280 |
| PDBID: | 7VAE |
| Chains: | HL_A |
| Organism: | Bos taurus, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.55 |
| Reference: | 10.1038/s41586-022-04845-4 |
| Antibody | |
| Antibody: | YN69202 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | bovine sodium taurocholate co-transporting polypeptide (NTCP) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7VAE_H|Chain B[auth H]|Fab heavy chain from antibody IgG clone number YN69202|Mus musculus (10090) EVQLQQSGPELVKPGASVKMSCKASGYTFTSYIIHWVKQKPGQGLEWIGYINPYNDGTKYNEKFKGKGTLTSDKSSSTAYMELSSLTSEDSAVYYCARSYYDGIPHYFDYWGQGTTLTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPSGPISTINPCPPCKECHKCPAPNLEGGPS |
Light Chain: L
Mutation: NULL
| >7VAE_L|Chain C[auth L]|Fab light chain from antibody IgG clone number YN69202|Mus musculus (10090) DIVMTQSPSSLAVSAGEKVTMSCKSSQSLFNSRTRRNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCKQSYYLLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: Q261A
| >7VAE_A|Chain A|Solute carrier family 10 (Sodium/bile acid cotransporter family), member 1|Bos taurus (9913) MEAFNESSPFNFSLPHNFGKRPTDRALSVILVIMLLTIMLSLGCTMEFSKIKAHFWRPKGLAVALVAQFGIMPLTAFGLGKFFQLNNVEALAILICGCSPGGNLSNVFALAIKGDMNLSIVMTTCSTFFALGMMPLLLYLYSRGIYDGSLKDKVPYGGIMISLILILIPCTIGIILKSKRPQYVRYVTKGGIILLLLCSVAVVVLSAINVGKSILFVMTPHLLATSSLMPFIGFLLGYLLSALFCLNGRCKRTVSMETGCANIQLCSTILNVTFPPEVIGPLFFFPLLYMIFQVGEGLLLVAIFRCYEKFKTPRDDTKMTENLYFQGDYKDDDDKHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 THR30 SER31 TYR32 TYR50 ASN52 TYR54 ASN55 LYS59 TYR100 TYR101 ASP102 GLY103 ILE104 PRO105 L: ASN31 SER32 ARG33 THR34 ARG35 ARG36 TYR38 TYR98 TYR99 LEU100 A: PHE77 GLY80 LYS81 GLN84 LEU85 ASN86 ASN87 VAL88 ALA90 ARG143 GLY144 ILE145 TYR146 ASP147 GLY148 LYS153 ASN271 VAL272 THR273 PHE274 PRO275 PRO276 GLU277 VAL278 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
