Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 528 |
| PDBID: | 3J8V |
| Chains: | FJ_D |
| Organism: | Human papillomavirus 16, Mus musculus |
| Method: | EM |
| Resolution (Å): | 13.90 |
| Reference: | 10.1016/j.virol.2015.04.016 |
| Antibody | |
| Antibody: | H16.14J Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Major capsid protein L1 from Human papillomavirus type 16 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >3J8V_F|Chain G[auth F], I[auth G], K[auth H], M[auth I]|H16.14J heavy chain|Mus musculus (10090) QLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIYPGDGATNYNGKFKGKATLTADKSSSTAFMQISSLTSEDSAVYFCARPYRYDGGVYAMDYWGQGTSVTVS |
Light Chain: J
Mutation: NULL
| >3J8V_J|Chain F[auth J], H[auth K], J[auth L], L[auth M]|H16.14J light chain|Mus musculus (10090) DIVMTQSPSYLSVSLGGRVTITCKASDHINNWLAWYQQKPGNAPRLLISGATSLETGVPSRFSGSGSGKDFTLSITSLQTEDVATYHCQQYWSTPLTFGAGTKLELKRA |
Antigen
Chain: D
Mutation: NULL
| >3J8V_D|Chain A, B, C, D, E|L1|Human papillomavirus type 16 (333760) AVVSTDEYVARTNIYYHAGTSRLLAVGHPYFPIKKPNNNKILVPKVSGLQYRVFRIHLPDPNKFGFPDTSFYNPDTQRLVWACVGVEVGRGQPLGVGISGHPLLNKLDDTENASAYAANAGVDNRECISMDYKQTQLCLIGCKPPIGEHWGKGSPCTQVAVQPGDCPPLELINTVIQDGDMVDTGFGAMDFTTLQANKSEVPLDICTSICKYPDYIKMVSEPYGDSLFFYLRREQMFVRHLFNRAGTVGENVPDDLYIKGSGSTANLASSNYFPTPSGSMVTSDAQIFNKPYWLQRAQGHNNGICWGNQLFVTVVDTTRSTNMSLCAAISTSETTYKNTNFKEYLRHGEEYDLQFIFQLCKITLTADVMTYIHSMNSTILEDWNFGLQPPPGGTLEDTYRFVTSQAIACQKHTPPAPKEDPLKKYTFWEVNLKEKFSADLDQFPLGRKFLLQLGL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| J: ASN30 TRP32 TRP92 THR94 F: SER30 TRP33 GLY53 LYS73 ARG97 TYR98 ASP99 D: ASN138 ALA139 GLN181 PRO182 THR266 VAL267 GLY268 ASN270 LYS278 GLY279 SER280 GLY281 SER282 ALA284 ASN285 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
