Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 526 |
| PDBID: | 3J70 |
| Chains: | SR_U |
| Organism: | Homo sapiens, Human immunodeficiency virus 1 |
| Method: | EM |
| Resolution (Å): | 20.00 |
| Reference: | 10.1016/j.str.2015.03.026 |
| Antibody | |
| Antibody: | 17b Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV_1 Envelope glycoprotein gp120 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: S
Mutation: NULL
| >3J70_S|Chain B, G[auth N], L[auth S]|monoclonal antibody 17b heavy chain|Homo sapiens (9606) DIQMTQSPSSVSASVGDRVTITCRASQDISTWLAWYQQKPGKAPKLLIYAASTLQSGVPSRFSGSGSGTDFSLTINSLQPEDFATYYCQQANSFFTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKKLYACEVTQGLSSPVTKSFNRGE |
Light Chain: R
Mutation: NULL
| >3J70_R|Chain A, F[auth M], K[auth R]|monoclonal antibody 17b light chain|Homo sapiens (9606) EVQLVQSGAEVKKPGATVKISCKASGYTFSDFYMYWVRQAPGKGLEWMGLIDEDADTMYAEKFRGRVTITADTSTDTGYLELRSEDTAVYYCAADPWELNNVWGQGTLVSVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNKPSNTKVDKKVEPK |
Antigen
Chain: U
Mutation: NULL
| >3J70_U|Chain D, I[auth P], N[auth U]|Envelope glycoprotein gp120|Human immunodeficiency virus 1 (11676) TEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFNISTSIRGKVQKEYAFFYKLDIIPIDNDTTSYKLTSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSVNFTDNAKTIIVQLNTSVEINCTRPNNNTRKRIRIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNNTLKQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNSTWFNSTWSTEGSNNTEGSDTITLPCRIKQIINMWQKVGKAMYAPPISGQIRCSSNITGLLLTRDGGNSNNESEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| R: ASP31 TYR33 TYR35 ASP52 GLU53 ASP54 ALA55 ASP56 THR57 MET58 ARG64 GLY65 ASP95 GLU98 LEU99 ASN100 S: ASP28 SER30 THR31 TRP32 ILE48 TYR49 ALA50 ALA51 SER52 THR53 LEU54 SER60 PHE62 SER63 GLY64 SER65 GLY66 SER67 PHE71 ALA91 U: VAL120 VAL200 THR202 ARG304 LYS305 ARG306 ILE309 GLN310 GLY312 PRO313 GLY314 ARG315 ALA316 PHE317 VAL318 THR319 ILE320 LYS322 ILE323 GLY324 ASN325 ARG327 ARG419 ILE420 LYS421 GLN422 ILE423 MET434 PRO437 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
