Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 5230 |
| PDBID: | 7UXL |
| Chains: | AB_R |
| Organism: | Homo sapiens, Plasmodium falciparum |
| Method: | XRD |
| Resolution (Å): | 2.86 |
| Reference: | 10.1016/j.immuni.2023.01.009 |
| Antibody | |
| Antibody: | RUPA-29 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasmodium falciparum gametocyte surface protein P45/48 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7UXL_A|Chain D[auth A]|RUPA-29 Fab Heavy chain|Homo sapiens (9606) QVHLVESGGGVVQPGRSLRLSCAASGFTFRSYGMHWVRLAPGKGLEWVTAIWYDGSKKHFADSVKGRFTISRDNSKNTLYLQMSSLRVEDTAVYYCARDFAHGAFYPVDWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: B
Mutation: NULL
| >7UXL_B|Chain E[auth B]|RUPA-29 Fab Lambda chain|Homo sapiens (9606) SYELTQPPSVSVSPGQTARITCSGDALPKKHAYWYQQKSGQAPVLVIYDDSKRPSGIPERFSGSSSGTMATLSISGAQVEDEAAYYCYSSDTSANYWVFGGGTKLTVLVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: R
Mutation: H18Y/G107L/I112V
| >7UXL_R|Chain C[auth R]|Gametocyte surface protein P45/48|Plasmodium falciparum (5833) EKKVIHGCNFSSNVSSKYTFTDSLDISLVDDSAHISCNVHLSEPKYNHLVGLNCPGDIIPDCFFQVYQPESEELEPSNIVYLDSQINIGDIEYYEDAEGDDKIKLFLIVGSVPKTTSFTCICKKDKKSAYMTVTIDSAGTKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: THR28 ARG30 SER31 TYR32 TYR52 ASP53 PHE96 ALA97 HIS98 B: LEU28 PRO29 LYS30 LYS31 HIS32 TYR34 TYR49 ASP50 ASP51 LYS53 SER66 THR93 SER94 R: ASP321 ASP347 ILE348 ILE349 PRO350 ASP351 PHE354 GLN355 TYR357 LEU364 GLU365 PRO366 SER367 ILE369 TYR371 ASP390 ILE411 LYS413 ASP415 LYS416 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
